3LU0

Molecular model of Escherichia coli core RNA polymerase

3LU0 の概要

• エントリーDOI: 10.2210/pdb3lu0/pdb
• 関連するPDBエントリー: 3LTI
• EMDBエントリー: 5169
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (6 entities in total)
• 機能のキーワード: e. coli rna polymerase, dna-directed rna polymerase, nucleotidyltransferase, transcription, transferase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 389693.73

構造登録者

Darst, S.A. (登録日: 2010-02-16, 公開日: 2010-09-29, 最終更新日: 2024-02-21)

主引用文献

Opalka, N.,Brown, J.,Lane, W.J.,Twist, K.A.,Landick, R.,Asturias, F.J.,Darst, S.A.
Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
Plos Biol., 8:e1000483-, 2010

PubMed Abstract: The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the details of E. coli transcription and its regulation, and therefore its full exploitation as a model system, has been hampered by the absence of high-resolution structural information on E. coli RNA polymerase (RNAP). We use a combination of approaches, including high-resolution X-ray crystallography, ab initio structural prediction, homology modeling, and single-particle cryo-electron microscopy, to generate complete atomic models of E. coli core RNAP and an E. coli RNAP ternary elongation complex. The detailed and comprehensive structural descriptions can be used to help interpret previous biochemical and genetic data in a new light and provide a structural framework for designing experiments to understand the function of the E. coli lineage-specific insertions and their role in the E. coli transcription program.

PubMed: 20856905
DOI: 10.1371/journal.pbio.1000483

実験手法

ELECTRON MICROSCOPY (11.2 Å)