3LSV

Structure of the A237F mutant of the pentameric ligand gated ion channel from Gloeobacter Violaceus

Summary for 3LSV

• Entry DOI: 10.2210/pdb3lsv/pdb
• Related: 3eam
• Descriptor: Ligand-gated ion channel (2 entities in total)
• Functional Keywords: plgic, channel, cys-loop, membrane protein, transport protein
• Biological source: Gloeobacter violaceus
• Total number of polymer chains: 5
• Total formula weight: 181839.22

Authors

Nury, H.,Corringer, P.J.,Delarue, M. (deposition date: 2010-02-13, release date: 2010-04-28, Last modification date: 2024-02-21)

Primary citation

Nury, H.,Poitevin, F.,Van Renterghem, C.,Changeux, J.P.,Corringer, P.J.,Delarue, M.,Baaden, M.
One-microsecond molecular dynamics simulation of channel gating in a nicotinic receptor homologue.
Proc.Natl.Acad.Sci.USA, 107:6275-6280, 2010

PubMed Abstract: Recently discovered bacterial homologues of eukaryotic pentameric ligand-gated ion channels, such as the Gloeobacter violaceus receptor (GLIC), are increasingly used as structural and functional models of signal transduction in the nervous system. Here we present a one-microsecond-long molecular dynamics simulation of the GLIC channel pH stimulated gating mechanism. The crystal structure of GLIC obtained at acidic pH in an open-channel form is equilibrated in a membrane environment and then instantly set to neutral pH. The simulation shows a channel closure that rapidly takes place at the level of the hydrophobic furrow and a progressively increasing quaternary twist. Two major events are captured during the simulation. They are initiated by local but large fluctuations in the pore, taking place at the top of the M2 helix, followed by a global tertiary relaxation. The two-step transition of the first subunit starts within the first 50 ns of the simulation and is followed at 450 ns by its immediate neighbor in the pentamer, which proceeds with a similar scenario. This observation suggests a possible two-step domino-like tertiary mechanism that takes place between adjacent subunits. In addition, the dynamical properties of GLIC described here offer an interpretation of the paradoxical properties of a permeable A13'F mutant whose crystal structure determined at 3.15 A shows a pore too narrow to conduct ions.

PubMed: 20308576
DOI: 10.1073/pnas.1001832107

Experimental method

X-RAY DIFFRACTION (3.15 Å)