3LS2

Crystal structure of an S-formylglutathione hydrolase from Pseudoalteromonas haloplanktis TAC125

3LS2 の概要

• エントリーDOI: 10.2210/pdb3ls2/pdb
• 分子名称: S-formylglutathione Hydrolase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: s-formylglutathione hydrolase, psychrophilic organism, pseudoalteromonas haloplanktis, hydrolase
• 由来する生物種: Pseudoalteromonas haloplanktis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 123804.74

構造登録者

Alterio, V.,De Simone, G. (登録日: 2010-02-12, 公開日: 2010-03-23, 最終更新日: 2023-09-06)

主引用文献

Alterio, V.,Aurilia, V.,Romanelli, A.,Parracino, A.,Saviano, M.,D'Auria, S.,De Simone, G.
Crystal structure of an S-formylglutathione hydrolase from Pseudoalteromonas haloplanktis TAC125.
Biopolymers, 93:669-677, 2010

PubMed Abstract: S-formylglutathione hydrolases (FGHs) constitute a family of ubiquitous enzymes which play a key role in formaldehyde detoxification both in prokaryotes and eukaryotes, catalyzing the hydrolysis of S-formylglutathione to formic acid and glutathione. While a large number of functional studies have been reported on these enzymes, few structural studies have so far been carried out. In this article we report on the functional and structural characterization of PhEst, a FGH isolated from the psychrophilic bacterium Pseudoalteromonas haloplanktis. According to our functional studies, this enzyme is able to efficiently hydrolyze several thioester substrates with very small acyl moieties. By contrast, the enzyme shows no activity toward substrates with bulky acyl groups. These data are in line with structural studies which highlight for this enzyme a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold. PhEst represents the first cold-adapted FGH structurally characterized to date; comparison with its mesophilic counterparts of known three-dimensional structure allowed to obtain useful insights into molecular determinants responsible for the ability of this psychrophilic enzyme to work at low temperature.

PubMed: 20209484
DOI: 10.1002/bip.21420

実験手法

X-RAY DIFFRACTION (2.2 Å)