3LOB

Crystal Structure of Flock House Virus calcium mutant

3LOB の概要

• エントリーDOI: 10.2210/pdb3lob/pdb
• 関連するPDBエントリー: 1F8V 1NOV 2Q23 2Q25 2Q26 2Z2Q
• 分子名称: Coat protein beta, Coat protein gamma, RNA (5'-R(*UP*UP*U*AP*UP*CP*UP*(P))-3'), ... (4 entities in total)
• 機能のキーワード: virus, flock house virus, rna, beta-barrel, jellyroll, aspartyl protease, capsid protein, hydrolase, protease, virion, icosahedral virus
• 由来する生物種: Flock house virus (FHV); 詳細
• 細胞内の位置: Capsid protein beta: Virion (Potential). Peptide gamma: Virion (Potential): P12870 P12870
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 134254.29

構造登録者

Johnson, J.E.,Banerjee, M.,Speir, J.A.,Huang, R. (登録日: 2010-02-03, 公開日: 2010-04-21, 最終更新日: 2024-11-20)

主引用文献

Banerjee, M.,Speir, J.A.,Kwan, M.H.,Huang, R.,Aryanpur, P.P.,Bothner, B.,Johnson, J.E.
Structure and function of a genetically engineered mimic of a nonenveloped virus entry intermediate.
J.Virol., 84:4737-4746, 2010

PubMed Abstract: Divalent metal ions are components of numerous icosahedral virus capsids. Flock House virus (FHV), a small RNA virus of the family Nodaviridae, was utilized as an accessible model system with which to address the effects of metal ions on capsid structure and on the biology of virus-host interactions. Mutations at the calcium-binding sites affected FHV capsid stability and drastically reduced virus infectivity, without altering the overall architecture of the capsid. The mutations also altered the conformation of gamma, a membrane-disrupting, virus-encoded peptide usually sequestered inside the capsid, by increasing its exposure under neutral pH conditions. Our data demonstrate that calcium binding is essential for maintaining a pH-based control on gamma exposure and host membrane disruption, and they reveal a novel rationale for the metal ion requirement during virus entry and infectivity. In the light of the phenotypes displayed by a calcium site mutant of FHV, we suggest that this mutant corresponds to an early entry intermediate formed in the endosomal pathway.

PubMed: 20164221
DOI: 10.1128/JVI.02670-09

実験手法

X-RAY DIFFRACTION (3.6 Å)