3LNI

Crystal structure of E-cadherin EC12 E89A

Summary for 3LNI

• Entry DOI: 10.2210/pdb3lni/pdb
• Related: 3LND 3LNE 3LNF 3LNG 3LNH 3LNJ
• Descriptor: Cadherin-1, CALCIUM ION (3 entities in total)
• Functional Keywords: cadherin, cell adhesion, cell junction, cell membrane, cleavage on pair of basic residues, glycoprotein, membrane, transmembrane
• Biological source: Mus musculus (mouse)
• Cellular location: Cell junction: P09803
• Total number of polymer chains: 2
• Total formula weight: 46646.13

Authors

Harrison, O.,Jin, X.,Shapiro, L. (deposition date: 2010-02-02, release date: 2010-03-02, Last modification date: 2023-09-06)

Primary citation

Harrison, O.J.,Bahna, F.,Katsamba, P.S.,Jin, X.,Brasch, J.,Vendome, J.,Ahlsen, G.,Carroll, K.J.,Price, S.R.,Honig, B.,Shapiro, L.
Two-step adhesive binding by classical cadherins.
Nat.Struct.Mol.Biol., 17:348-357, 2010

PubMed Abstract: Crystal structures of classical cadherins have revealed two dimeric configurations. In the first, N-terminal beta-strands of EC1 domains 'swap' between partner molecules. The second configuration (the 'X dimer'), also observed for T-cadherin, is mediated by residues near the EC1-EC2 calcium binding sites, and N-terminal beta-strands of partner EC1 domains, though held adjacent, do not swap. Here we show that strand-swapping mutants of type I and II classical cadherins form X dimers. Mutant cadherins impaired for X-dimer formation show no binding in short-time frame surface plasmon resonance assays, but in long-time frame experiments, they have homophilic binding affinities close to that of wild type. Further experiments show that exchange between monomers and dimers is slowed in these mutants. These results reconcile apparently disparate results from prior structural studies and suggest that X dimers are binding intermediates that facilitate the formation of strand-swapped dimers.

PubMed: 20190754
DOI: 10.1038/nsmb.1784

Experimental method

X-RAY DIFFRACTION (2.3 Å)