3LKU

Crystal structure of S. cerevisiae Get4 in complex with an N-terminal fragment of Get5

3LKU の概要

• エントリーDOI: 10.2210/pdb3lku/pdb
• 分子名称: UPF0363 protein YOR164C, Ubiquitin-like protein MDY2, PROLINE, ... (4 entities in total)
• 機能のキーワード: alpha helical repeat, tpr-like, protein binding
• 由来する生物種: Saccharomyces cerevisiae (yeast); 詳細
• 細胞内の位置: Cytoplasm: Q12125; Cytoplasm, cytosol: Q12285
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 121776.64

構造登録者

Chartron, J.W.,Clemons Jr., W.M. (登録日: 2010-01-27, 公開日: 2010-06-23, 最終更新日: 2024-10-30)

主引用文献

Chartron, J.W.,Suloway, C.J.,Zaslaver, M.,Clemons, W.M.
Structural characterization of the Get4/Get5 complex and its interaction with Get3.
Proc.Natl.Acad.Sci.USA, 107:12127-12132, 2010

PubMed Abstract: The recently elucidated Get proteins are responsible for the targeted delivery of the majority of tail-anchored (TA) proteins to the endoplasmic reticulum. Get4 and Get5 have been identified in the early steps of the pathway mediating TA substrate delivery to the cytoplasmic targeting factor Get3. Here we report a crystal structure of Get4 and an N-terminal fragment of Get5 from Saccharomyces cerevisae. We show Get4 and Get5 (Get4/5) form an intimate complex that exists as a dimer (two copies of Get4/5) mediated by the C-terminus of Get5. We further demonstrate that Get3 specifically binds to a conserved surface on Get4 in a nucleotide dependent manner. This work provides further evidence for a model in which Get4/5 operates upstream of Get3 and mediates the specific delivery of a TA substrate.

PubMed: 20554915
DOI: 10.1073/pnas.1006036107

実験手法

X-RAY DIFFRACTION (2.8 Å)