3LFK

A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators

Summary for 3LFK

• Entry DOI: 10.2210/pdb3lfk/pdb
• Descriptor: MarR Like Protein, TVG0766549, SULFATE ION, CITRIC ACID, ... (4 entities in total)
• Functional Keywords: multiple antibiotic resistance regulator, transcriptional regulator, winged helix-turn-helix motif, mechanosensitive channel, unknown function
• Biological source: Thermoplasma volcanium
• Total number of polymer chains: 4
• Total formula weight: 58915.80

Authors

Liu, Z.,Walton, T.A.,Rees, D.C. (deposition date: 2010-01-17, release date: 2010-04-14, Last modification date: 2024-02-21)

Primary citation

Liu, Z.,Walton, T.A.,Rees, D.C.
A reported archaeal mechanosensitive channel is a structural homolog of MarR-like transcriptional regulators.
Protein Sci., 19:808-814, 2010

PubMed Abstract: Several archaeal mechanosensitive (MS) channels have been reported, including one from Thermoplasma volcanium designated MscTV. Here, we report the crystal structure of MscTV at 1.6-A resolution. Unexpectedly, MscTV was found to be a water-soluble protein exhibiting a winged helix-turn-helix (wHTH) motif, which is the signature of the MarR (multiple antibiotic resistance regulator) family of transcriptional regulators. A cell-based osmotic downshock functional assay demonstrated that MscTV was unable to protect a knockout strain of Escherichia coli from hypoosmotic shock, further indicating that it does not function as a MS channel. We propose this protein be renamed MLPTv for MarR-like protein from T. volcanium.

PubMed: 20162616
DOI: 10.1002/pro.360

Experimental method

X-RAY DIFFRACTION (1.6 Å)