3LA5

X-ray crystal structure of mc6 RNA Riboswitch bound to azacytosine

Summary for 3LA5

• Entry DOI: 10.2210/pdb3la5/pdb
• Related: 1Y26
• Descriptor: Adenosine RIboswitch, MAGNESIUM ION, 6-amino-1,3,5-triazin-2(1H)-one, ... (4 entities in total)
• Functional Keywords: rna, riboswitch, gene regulation, synthetic biology
• Biological source: Bacillus subtilis
• Total number of polymer chains: 1
• Total formula weight: 23055.57

Authors

Dunstan, M.S.,Leys, D. (deposition date: 2010-01-06, release date: 2010-01-26, Last modification date: 2023-11-01)

Primary citation

Dixon, N.,Duncan, J.N.,Geerlings, T.,Dunstan, M.S.,McCarthy, J.E.G.,Leys, D.,Micklefield, J.
Reengineering orthogonally selective riboswitches
Proc.Natl.Acad.Sci.USA, 107:2830-2835, 2010

PubMed Abstract: The ability to independently control the expression of multiple genes by addition of distinct small-molecule modulators has many applications from synthetic biology, functional genomics, pharmaceutical target validation, through to gene therapy. Riboswitches are relatively simple, small-molecule-dependent, protein-free, mRNA genetic switches that are attractive targets for reengineering in this context. Using a combination of chemical genetics and genetic selection, we have developed riboswitches that are selective for synthetic "nonnatural" small molecules and no longer respond to the natural intracellular ligands. The orthogonal selectivity of the riboswitches is also demonstrated in vitro using isothermal titration calorimetry and x-ray crystallography. The riboswitches allow highly responsive, dose-dependent, orthogonally selective, and dynamic control of gene expression in vivo. It is possible that this approach may be further developed to reengineer other natural riboswitches for application as small-molecule responsive genetic switches in both prokaryotes and eukaryotes.

PubMed: 20133756
DOI: 10.1073/pnas.0911209107

Experimental method

X-RAY DIFFRACTION (1.7 Å)