3L51

Crystal Structure of the Mouse Condensin Hinge Domain

Summary for 3L51

• Entry DOI: 10.2210/pdb3l51/pdb
• Descriptor: Structural maintenance of chromosomes protein 2, Structural maintenance of chromosomes protein 4, GLYCEROL, ... (4 entities in total)
• Functional Keywords: structural maintenance of chromosomes (smc), hinge domain, cell cycle, cell division, cytoplasm, dna condensation, mitosis, nucleus
• Biological source: Mus musculus (mouse); More
• Cellular location: Nucleus (By similarity): Q8CG48 Q8CG47
• Total number of polymer chains: 2
• Total formula weight: 37484.04

Authors

Griese, J.J.,Hopfner, K.-P. (deposition date: 2009-12-21, release date: 2010-02-16, Last modification date: 2011-07-13)

Primary citation

Griese, J.J.,Witte, G.,Hopfner, K.-P.
Structure and DNA binding activity of the mouse condensin hinge domain highlight common and diverse features of SMC proteins
Nucleic Acids Res., 38:3454-3465, 2010

PubMed Abstract: Structural Maintenance of Chromosomes (SMC) proteins are vital for a wide range of processes including chromosome structure and dynamics, gene regulation and DNA repair. Eukaryotes have three SMC complexes, consisting of heterodimeric pairs of six different SMC proteins along with several specific regulatory subunits. In addition to their other functions, all three SMC complexes play distinct roles in DNA repair. Cohesin (SMC1-SMC3) is involved in DNA double-strand break repair, condensin (SMC2-SMC4) participates in single-strand break (SSB) repair, and the SMC5-SMC6 complex functions in various DNA repair pathways. SMC proteins consist of N- and C-terminal domains that fold back onto each other to create an ATPase 'head' domain, connected to a central 'hinge' domain via long coiled-coils. The hinge domain mediates dimerization of SMC proteins and binds DNA, but it is not clear to what purpose this activity serves. We studied the structure and function of the condensin hinge domain from mouse. While the SMC hinge domain structure is largely conserved from prokaryotes to eukaryotes, its function seems to have diversified throughout the course of evolution. The condensin hinge domain preferentially binds single-stranded DNA. We propose that this activity plays a role in the SSB repair function of the condensin complex.

PubMed: 20139420
DOI: 10.1093/nar/gkq038

Experimental method

X-RAY DIFFRACTION (1.506 Å)