3L1V

Crystal structure of GmhB from E. coli in complex with calcium and phosphate.

3L1V の概要

• エントリーDOI: 10.2210/pdb3l1v/pdb
• 関連するPDBエントリー: 3L1U
• 分子名称: D,D-heptose 1,7-bisphosphate phosphatase, ZINC ION, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: lps biosynthesis, sugar phosphatase, zinc, heptose, carbohydrate metabolism, cytoplasm, hydrolase, lipopolysaccharide biosynthesis
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm : P63228
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47382.13

構造登録者

Sugiman-Marangos, S.N.,Junop, M.S. (登録日: 2009-12-14, 公開日: 2010-01-05, 最終更新日: 2023-09-06)

主引用文献

Taylor, P.L.,Sugiman-Marangos, S.,Zhang, K.,Valvano, M.A.,Wright, G.D.,Junop, M.S.
Structural and kinetic characterization of the LPS biosynthetic enzyme D-alpha,beta-D-heptose-1,7-bisphosphate phosphatase (GmhB) from Escherichia coli.
Biochemistry, 49:1033-1041, 2010

PubMed Abstract: Lipopolysaccharide is a major component of the outer membrane of gram-negative bacteria and provides a permeability barrier to many commonly used antibiotics. ADP-heptose residues are an integral part of the LPS inner core, and mutants deficient in heptose biosynthesis demonstrate increased membrane permeability. The heptose biosynthesis pathway involves phosphorylation and dephosphorylation steps not found in other pathways for the synthesis of nucleotide sugar precursors. Consequently, the heptose biosynthetic pathway has been marked as a novel target for antibiotic adjuvants, which are compounds that facilitate and potentiate antibiotic activity. D-alpha,beta-D-heptose-1,7-bisphosphate phosphatase (GmhB) catalyzes the third essential step of LPS heptose biosynthesis. This study describes the first crystal structure of GmhB and enzymatic analysis of the protein. Structure-guided mutations followed by steady state kinetic analysis, together with established precedent for HAD phosphatases, suggest that GmhB functions through a phosphoaspartate intermediate. This study provides insight into the structure-function relationship of GmhB, a new target for combatting gram-negative bacterial infection.

PubMed: 20050699
DOI: 10.1021/bi901780j

実験手法

X-RAY DIFFRACTION (1.954 Å)