3KZ7

C-terminal domain of Murine FKBP25 rapamycin complex

3KZ7 の概要

• エントリーDOI: 10.2210/pdb3kz7/pdb
• 関連するPDBエントリー: 1PBK
• 分子名称: FK506-binding protein 3, RAPAMYCIN IMMUNOSUPPRESSANT DRUG (3 entities in total)
• 機能のキーワード: fkpb ppiase rapamycin, isomerase, nucleus, phosphoprotein, rotamase, isomerase-inhibitor complex, isomerase/inhibitor
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Nucleus (By similarity): Q62446
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14198.51

構造登録者

Stura, E.A.,Galat, A. (登録日: 2009-12-08, 公開日: 2010-12-15, 最終更新日: 2023-11-01)

主引用文献

Galat, A.,Thai, R.,Stura, E.A.
Diversified targets of FKBP25 and its complex with rapamycin.
Int.J.Biol.Macromol., 69:344-352, 2014

PubMed Abstract: FKBP25 is a member of the super-family of peptidylprolyl cis/trans isomerases, which is a high affinity binder for the immunosuppressive antibiotic rapamycin (Rpm). FKBP25 isolated from natural sources, its recombinant murine homologue (mFKBP25) and their complexes with rapamycin bind to diverse DNAs, RNAs and heparin affinity beads. The recombinant mFKBP25/rapamycin complex binds to several proteins including the calcineurin-A/calcineurin-B/calmodulin complex and to elongation factor 1β. We solved the X-ray structure of the C-terminal domain of mFKBP25 bound to rapamycin that has a higher resolution than of its human counterpart, and which clearly illustrates that the positively charged 40s loop is an epitope of the FK506-like binding domain (FKBD) for interactions with various biopolymers.

PubMed: 24879919
DOI: 10.1016/j.ijbiomac.2014.05.060

実験手法

X-RAY DIFFRACTION (1.95 Å)