3KYQ

Lipid-induced Conformational Switch Controls Fusion Activity of Longin Domain SNARE Ykt6

3KYQ の概要

• エントリーDOI: 10.2210/pdb3kyq/pdb
• 分子名称: Synaptobrevin homolog YKT6, SULFATE ION, dodecyl 2-(trimethylammonio)ethyl phosphate, ... (4 entities in total)
• 機能のキーワード: v-snare homolog, lipid binding, cytoplasmic vesicle, er-golgi transport, golgi apparatus, lipoprotein, palmitate, prenylation, protein transport, transferase, transport
• 由来する生物種: Rattus norvegicus (Rat)
• 細胞内の位置: Cytoplasm, cytosol: Q5EGY4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23055.24

構造登録者

Yu, J.,Wen, W.Y.,Zhang, M.J. (登録日: 2009-12-07, 公開日: 2010-03-02, 最終更新日: 2023-11-01)

主引用文献

Wen, W.Y.,Yu, J.,Pan, L.F.,Wei, Z.Y.,Weng, J.W.,Wang, W.N.,Ong, Y.S.,Tran, T.H.T.,Hong, W.J.,Zhang, M.J.
Lipid-Induced Conformational Switch Controls Fusion Activity of Longin Domain SNARE Ykt6
Mol.Cell, 37:383-395, 2010

PubMed Abstract: While most SNAREs are permanently anchored to membranes by their transmembrane domains, the dually lipidated SNARE Ykt6 is found both on intracellular membranes and in the cytosol. The cytosolic Ykt6 is inactive due to the autoinhibition of the SNARE core by its longin domain, although the molecular basis of this inhibition is unknown. Here, we demonstrate that unlipidated Ykt6 adopts multiple conformations, with a small population in the closed state. The structure of Ykt6 in complex with a fatty acid suggests that, upon farnesylation, the Ykt6 SNARE core forms four alpha helices that wrap around the longin domain, forming a dominantly closed conformation. The fatty acid, buried in a hydrophobic groove formed between the longin domain and its SNARE core, is essential for maintaining the autoinhibited conformation of Ykt6. Our study reveals that the posttranslationally attached farnesyl group can actively regulate Ykt6 fusion activity in addition to its anticipated membrane-anchoring role.

PubMed: 20159557
DOI: 10.1016/j.molcel.2010.01.024

実験手法

X-RAY DIFFRACTION (2.443 Å)