3KXB

Structural characterization of H3K56Q nucleosomes and nucleosomal arrays

3KXB の概要

• エントリーDOI: 10.2210/pdb3kxb/pdb
• 関連するPDBエントリー: 1AOI
• 分子名称: Histone H3.2, Histone H4, Histone H2A, ... (6 entities in total)
• 機能のキーワード: nucleosome, transcription, transcription-dna complex, transcription/dna
• 由来する生物種: Xenopus laevis (clawed frog,common platanna,platanna); 詳細
• 細胞内の位置: Nucleus: P84233 P62799 P02281; Nucleus (By similarity): Q6AZJ8
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 198249.86

構造登録者

Clark, N.J.,Lilyestrom, W.G. (登録日: 2009-12-02, 公開日: 2010-06-23, 最終更新日: 2023-09-06)

主引用文献

Watanabe, S.,Resch, M.,Lilyestrom, W.,Clark, N.,Hansen, J.C.,Peterson, C.,Luger, K.
Structural characterization of H3K56Q nucleosomes and nucleosomal arrays.
Biochim.Biophys.Acta, 1799:480-486, 2010

PubMed Abstract: The post-translational modification of histones is a key mechanism for the modulation of DNA accessibility. Acetylated lysine 56 in histone H3 is associated with nucleosome assembly during replication and DNA repair, and is thus likely to predominate in regions of chromatin containing nucleosome-free regions. Here we show by X-ray crystallography that mutation of H3 lysine 56 to glutamine (to mimic acetylation) or glutamate (to cause a charge reversal) has no detectable effects on the structure of the nucleosome. At the level of higher order chromatin structure, the K to Q substitution has no effect on the folding of model nucleosomal arrays in cis, regardless of the degree of nucleosome density. In contrast, defects in array-array interactions in trans ('oligomerization') are selectively observed for mutant H3 lysine 56 arrays that contain nucleosome-free regions. Our data suggests that H3K56 acetylation is one of the molecular mechanisms employed to keep chromatin with nucleosome-free regions accessible to the DNA replication and repair machinery.

PubMed: 20100606
DOI: 10.1016/j.bbagrm.2010.01.009

実験手法

X-RAY DIFFRACTION (3.2 Å)