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3KWQ

Structural characterization of H3K56Q nucleosomes and nucleosomal arrays

3KWQ の概要
エントリーDOI10.2210/pdb3kwq/pdb
関連するPDBエントリー1AOI
分子名称Histone H3.2, Histone H4, Histone H2A, ... (6 entities in total)
機能のキーワードnucleosome transcription k56 mutation, acetylation, chromosomal protein, dna-binding, methylation, nucleosome core, nucleus, phosphoprotein, isopeptide bond, ubl conjugation, structural protein-dna complex, structural protein/dna
由来する生物種Xenopus laevis (clawed frog,common platanna,platanna)
詳細
細胞内の位置Nucleus: P84233 P62799 P02281
Nucleus (By similarity): Q6AZJ8
タンパク質・核酸の鎖数10
化学式量合計176135.75
構造登録者
Lilyestrom, W.G.,Clark, N. (登録日: 2009-12-01, 公開日: 2010-05-12, 最終更新日: 2023-09-06)
主引用文献Watanabe, S.,Resch, M.,Lilyestrom, W.,Clark, N.,Hansen, J.C.,Peterson, C.,Luger, K.
Structural characterization of H3K56Q nucleosomes and nucleosomal arrays.
Biochim.Biophys.Acta, 1799:480-486,
Cited by
PubMed Abstract: The post-translational modification of histones is a key mechanism for the modulation of DNA accessibility. Acetylated lysine 56 in histone H3 is associated with nucleosome assembly during replication and DNA repair, and is thus likely to predominate in regions of chromatin containing nucleosome-free regions. Here we show by X-ray crystallography that mutation of H3 lysine 56 to glutamine (to mimic acetylation) or glutamate (to cause a charge reversal) has no detectable effects on the structure of the nucleosome. At the level of higher order chromatin structure, the K to Q substitution has no effect on the folding of model nucleosomal arrays in cis, regardless of the degree of nucleosome density. In contrast, defects in array-array interactions in trans ('oligomerization') are selectively observed for mutant H3 lysine 56 arrays that contain nucleosome-free regions. Our data suggests that H3K56 acetylation is one of the molecular mechanisms employed to keep chromatin with nucleosome-free regions accessible to the DNA replication and repair machinery.
PubMed: 20100606
DOI: 10.1016/j.bbagrm.2010.01.009
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (3.5 Å)
構造検証レポート
Validation report summary of 3kwq
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-12-25に公開中

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