3KNS

Bacillus cereus metallo-beta-lactamase Cys221Asp mutant, 20 mM Zn(II)

3KNS の概要

• エントリーDOI: 10.2210/pdb3kns/pdb
• 関連するPDBエントリー: 1bc2 1bvt 3knr
• 分子名称: Beta-lactamase 2, ZINC ION, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: metallo-beta-lactamase, zn-dependent hydrolase, antibiotic resistance, hydrolase, metal-binding
• 由来する生物種: Bacillus cereus
• 細胞内の位置: Periplasm : P04190
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101762.19

構造登録者

Medrano Martin, F.J.,Gonzalez, J.M.,Vila, A.J. (登録日: 2009-11-12, 公開日: 2010-11-24, 最終更新日: 2024-02-21)

主引用文献

Gonzalez, J.M.,Meini, M.R.,Tomatis, P.E.,Medrano Martin, F.J.,Cricco, J.A.,Vila, A.J.
Metallo-beta-lactamases withstand low Zn(II) conditions by tuning metal-ligand interactions.
Nat.Chem.Biol., 8:698-700, 2012

PubMed Abstract: A number of multiresistant bacterial pathogens inactivate antibiotics by producing Zn(II)-dependent β-lactamases. We show that metal uptake leading to an active dinuclear enzyme in the periplasmic space of Gram-negative bacteria is ensured by a cysteine residue, an unusual metal ligand in oxidizing environments. Kinetic, structural and affinity data show that such Zn(II)-cysteine interaction is an adaptive trait that tunes the metal binding affinity, thus enabling antibiotic resistance at restrictive Zn(II) concentrations.

PubMed: 22729148
DOI: 10.1038/nchembio.1005

実験手法

X-RAY DIFFRACTION (1.58 Å)