3KN1

Crystal Structure of Golgi Phosphoprotein 3 N-term Truncation Variant

Summary for 3KN1

• Entry DOI: 10.2210/pdb3kn1/pdb
• Related: 2ZIH 2ZII
• Descriptor: Golgi phosphoprotein 3, SULFATE ION (3 entities in total)
• Functional Keywords: beta hairpin, phosphoinositide binding domain, cell membrane, cytoplasm, endosome, golgi apparatus, membrane, mitochondrion, phosphoprotein, protein binding
• Biological source: Homo sapiens (human)
• Cellular location: Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side: Q9H4A6
• Total number of polymer chains: 1
• Total formula weight: 29368.95

Authors

Schmitz, K.R.,Bessman, N.J.,Setty, T.G.,Ferguson, K.M. (deposition date: 2009-11-11, release date: 2009-12-15, Last modification date: 2023-11-22)

Primary citation

Wood, C.S.,Schmitz, K.R.,Bessman, N.J.,Setty, T.G.,Ferguson, K.M.,Burd, C.G.
PtdIns4P recognition by Vps74/GOLPH3 links PtdIns 4-kinase signaling to retrograde Golgi trafficking.
J.Cell Biol., 187:967-975, 2009

PubMed Abstract: Targeting and retention of resident integral membrane proteins of the Golgi apparatus underly the function of the Golgi in glycoprotein and glycolipid processing and sorting. In yeast, steady-state Golgi localization of multiple mannosyltransferases requires recognition of their cytosolic domains by the peripheral Golgi membrane protein Vps74, an orthologue of human GOLPH3/GPP34/GMx33/MIDAS (mitochondrial DNA absence sensitive factor). We show that targeting of Vps74 and GOLPH3 to the Golgi apparatus requires ongoing synthesis of phosphatidylinositol (PtdIns) 4-phosphate (PtdIns4P) by the Pik1 PtdIns 4-kinase and that modulation of the levels and cellular location of PtdIns4P leads to mislocalization of these proteins. Vps74 and GOLPH3 bind specifically to PtdIns4P, and a sulfate ion in a crystal structure of GOLPH3 indicates a possible phosphoinositide-binding site that is conserved in Vps74. Alterations in this site abolish phosphoinositide binding in vitro and Vps74 function in vivo. These results implicate Pik1 signaling in retention of Golgi-resident proteins via Vps74 and show that GOLPH3 family proteins are effectors of Golgi PtdIns 4-kinases.

PubMed: 20026658
DOI: 10.1083/jcb.200909063

Experimental method

X-RAY DIFFRACTION (2.9 Å)