3KIP

Crystal structure of type-II 3-dehydroquinase from C. albicans

Summary for 3KIP

Entry DOI	10.2210/pdb3kip/pdb
Descriptor	3-dehydroquinase, type II, SULFATE ION, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
Functional Keywords	lyase
Biological source	Candida albicans (Yeast)
Total number of polymer chains	24
Total formula weight	446628.51
Authors	Trapani, S.,Schoehn, G.,Navaza, J.,Abergel, C. (deposition date: 2009-11-02, release date: 2010-05-12, Last modification date: 2024-04-03)
Primary citation	Trapani, S.,Schoehn, G.,Navaza, J.,Abergel, C. Macromolecular crystal data phased by negative-stained electron-microscopy reconstructions. Acta Crystallogr.,Sect.D, 66:514-521, 2010 Cited by PubMed Abstract: The combination of transmission electron microscopy with X-ray diffraction data is usually limited to relatively large particles. Here, the approach is continued one step further by utilizing negative staining, a technique that is of wider applicability than cryo-electron microscopy, to produce models of medium-size proteins suitable for molecular replacement. The technique was used to solve the crystal structure of the dodecameric type II dehydroquinase enzyme from Candida albicans (approximately 190 kDa) and that of the orthologous Streptomyces coelicolor protein. PubMed: 20445226 DOI: 10.1107/S0907444910002763 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.95 Å)

Structure validation