3KIP

Crystal structure of type-II 3-dehydroquinase from C. albicans

3KIP の概要

• エントリーDOI: 10.2210/pdb3kip/pdb
• 分子名称: 3-dehydroquinase, type II, SULFATE ION, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• 機能のキーワード: lyase
• 由来する生物種: Candida albicans (Yeast)
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 446628.51

構造登録者

Trapani, S.,Schoehn, G.,Navaza, J.,Abergel, C. (登録日: 2009-11-02, 公開日: 2010-05-12, 最終更新日: 2024-04-03)

主引用文献

Trapani, S.,Schoehn, G.,Navaza, J.,Abergel, C.
Macromolecular crystal data phased by negative-stained electron-microscopy reconstructions.
Acta Crystallogr.,Sect.D, 66:514-521, 2010

PubMed Abstract: The combination of transmission electron microscopy with X-ray diffraction data is usually limited to relatively large particles. Here, the approach is continued one step further by utilizing negative staining, a technique that is of wider applicability than cryo-electron microscopy, to produce models of medium-size proteins suitable for molecular replacement. The technique was used to solve the crystal structure of the dodecameric type II dehydroquinase enzyme from Candida albicans (approximately 190 kDa) and that of the orthologous Streptomyces coelicolor protein.

PubMed: 20445226
DOI: 10.1107/S0907444910002763

実験手法

X-RAY DIFFRACTION (2.95 Å)