3KIC

Crystal structure of adeno-associated virus serotype 3B

3KIC の概要

• エントリーDOI: 10.2210/pdb3kic/pdb
• 関連するPDBエントリー: 3KIE
• 分子名称: Capsid protein VP1, 2'-DEOXYADENOSINE-5'-MONOPHOSPHATE (2 entities in total)
• 機能のキーワード: virus, capsid, parvovirus, icosahedral virus, beta barrel, single-stranded, dependovirus
• 由来する生物種: Adeno-associated virus 3B
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 1646368.82

構造登録者

Lerch, T.F.,Xie, Q.,Chapman, M.S. (登録日: 2009-11-01, 公開日: 2010-04-28, 最終更新日: 2023-09-06)

主引用文献

Lerch, T.F.,Xie, Q.,Chapman, M.S.
The structure of adeno-associated virus serotype 3B (AAV-3B): insights into receptor binding and immune evasion.
Virology, 403:26-36, 2010

PubMed Abstract: Adeno-associated viruses (AAVs) are leading candidate vectors for human gene therapy. AAV serotypes have broad cellular tropism and use a variety of cellular receptors. AAV serotype 3 binds to heparan sulfate proteoglycan prior to cell entry and is serologically distinct from other serotypes. The capsid features that distinguish AAV-3B from other serotypes are poorly understood. The structure of AAV-3B has been determined to 2.6A resolution from twinned crystals of an infectious virus. The most distinctive structural features are located in regions implicated in receptor and antibody binding, providing insights into the cell entry mechanisms and antigenic nature of AAVs. We show that AAV-3B has a lower affinity for heparin than AAV-2, which can be rationalized by the distinct features of the AAV-3B capsid. The structure of AAV-3B provides an additional foundation for the future engineering of improved gene therapy vectors with modified receptor binding or antigenic characteristics.

PubMed: 20444480
DOI: 10.1016/j.virol.2010.03.027

実験手法

X-RAY DIFFRACTION (2.603 Å)