3KHI

Crystal structure of a Putative Metal-dependent Hydrolase (YP_001336084.1) from Klebsiella pneumoniae subsp. pneumoniae MGH 78578 at 1.95 A resolution

3KHI の概要

• エントリーDOI: 10.2210/pdb3khi/pdb
• 分子名称: Putative Metal-dependent Hydrolase, ZINC ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: a putative metal-dependent hydrolase, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, hydrolase
• 由来する生物種: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31120.28

構造登録者

Joint Center for Structural Genomics (JCSG) (登録日: 2009-10-30, 公開日: 2009-11-10, 最終更新日: 2024-10-09)

主引用文献

Xu, Q.,Gohler, A.K.,Kosfeld, A.,Carlton, D.,Chiu, H.J.,Klock, H.E.,Knuth, M.W.,Miller, M.D.,Elsliger, M.A.,Deacon, A.M.,Godzik, A.,Lesley, S.A.,Jahreis, K.,Wilson, I.A.
The Structure of Mlc Titration Factor A (MtfA/YeeI) Reveals a Prototypical Zinc Metallopeptidase Related to Anthrax Lethal Factor.
J.Bacteriol., 194:2987-2999, 2012

PubMed Abstract: MtfA of Escherichia coli (formerly YeeI) was previously identified as a regulator of the phosphoenolpyruvate (PEP)-dependent:glucose phosphotransferase system. MtfA homolog proteins are highly conserved, especially among beta- and gammaproteobacteria. We determined the crystal structures of the full-length MtfA apoenzyme from Klebsiella pneumoniae and its complex with zinc (holoenzyme) at 2.2 and 1.95 Å, respectively. MtfA contains a conserved H(149)E(150)XXH(153)+E(212)+Y(205) metallopeptidase motif. The presence of zinc in the active site induces significant conformational changes in the region around Tyr205 compared to the conformation of the apoenzyme. Additionally, the zinc-bound MtfA structure is in a self-inhibitory conformation where a region that was disordered in the unliganded structure is now observed in the active site and a nonproductive state of the enzyme is formed. MtfA is related to the catalytic domain of the anthrax lethal factor and the Mop protein involved in the virulence of Vibrio cholerae, with conservation in both overall structure and in the residues around the active site. These results clearly provide support for MtfA as a prototypical zinc metallopeptidase (gluzincin clan).

PubMed: 22467785
DOI: 10.1128/JB.00038-12

実験手法

X-RAY DIFFRACTION (1.95 Å)