3KCU

Structure of formate channel

3KCU の概要

• エントリーDOI: 10.2210/pdb3kcu/pdb
• 関連するPDBエントリー: 3KCV
• 分子名称: Probable formate transporter 1, 2-(6-(2-CYCLOHEXYLETHOXY)-TETRAHYDRO-4,5-DIHYDROXY-2(HYDROXYMETHYL)-2H-PYRAN-3-YLOXY)-TETRAHYDRO-6(HYDROXYMETHYL)-2H-PY RAN-3,4,5-TRIOL (3 entities in total)
• 機能のキーワード: tcdb id 2.a.44.1.1, transporter, channel, formate, cell inner membrane, cell membrane, membrane, transmembrane, transport, transport protein
• 由来する生物種: Escherichia coli O157:H7
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein (By similarity): P0AC25
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 155515.52

構造登録者

Wang, Y.,Huang, Y.,Wang, J.,Yan, N.,Shi, Y. (登録日: 2009-10-22, 公開日: 2009-12-01, 最終更新日: 2024-03-20)

主引用文献

Wang, Y.,Huang, Y.,Wang, J.,Cheng, C.,Huang, W.,Lu, P.,Xu, Y.-N.,Wang, P.,Yan, N.,Shi, Y.
Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel
Nature, 462:467-472, 2009

PubMed Abstract: FocA is a representative member of the formate-nitrite transporter family, which transports short-chain acids in bacteria, archaea, fungi, algae and parasites. The structure and transport mechanism of the formate-nitrite transporter family remain unknown. Here we report the crystal structure of Escherichia coli FocA at 2.25 A resolution. FocA forms a symmetric pentamer, with each protomer consisting of six transmembrane segments. Despite a lack of sequence homology, the overall structure of the FocA protomer closely resembles that of aquaporin and strongly argues that FocA is a channel, rather than a transporter. Structural analysis identifies potentially important channel residues, defines the channel path and reveals two constriction sites. Unlike aquaporin, FocA is impermeable to water but allows the passage of formate. A structural and biochemical investigation provides mechanistic insights into the channel activity of FocA.

PubMed: 19940917
DOI: 10.1038/nature08610

実験手法

X-RAY DIFFRACTION (2.243 Å)