3KCP

Crystal structure of interacting Clostridium thermocellum multimodular components

3KCP の概要

• エントリーDOI: 10.2210/pdb3kcp/pdb
• 分子名称: Cellulosomal-scaffolding protein A, Cellulosome anchoring protein, cohesin region, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: cohesin, dockerin, x-module, cellulosome, carbohydrate metabolism, cell wall biogenesis/degradation, cellulose degradation, glycoprotein, polysaccharide degradation, secreted, structural protein
• 由来する生物種: Clostridium thermocellum ATCC 27405; 詳細
• 細胞内の位置: Secreted: Q06851
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55341.75

構造登録者

Adams, J.J.,Currie, M.A.,Bayer, E.A.,Jia, Z.,Smith, S.P. (登録日: 2009-10-21, 公開日: 2010-02-09, 最終更新日: 2024-02-21)

主引用文献

Adams, J.J.,Currie, M.A.,Ali, S.,Bayer, E.A.,Jia, Z.,Smith, S.P.
Insights into Higher-Order Organization of the Cellulosome Revealed by a Dissect-and-Build Approach: Crystal Structure of Interacting Clostridium thermocellum Multimodular Components
J.Mol.Biol., 396:833-839, 2010

PubMed Abstract: Cellulosomes are large, multienzyme, plant cell wall-degrading protein complexes found affixed to the surface of a variety of anaerobic microbes. The core of the cellulosome is a noncatalytic scaffoldin protein, which contains several type-I cohesin modules that bind type-I dockerin-containing enzymatic subunits, a cellulose-binding module, an X module, and a type-II dockerin that interacts with type-II cohesin-containing cell surface proteins. The unique arrangement of the enzymatic subunits in the cellulosome complex, made possible by the scaffoldin subunit, promotes enhanced substrate degradation relative to the enzymes free in solution. Despite representative high-resolution structures of all of the individual modules of the cellulosome, this mechanism of enzymatic synergy remains poorly understood. Consequently, a model of the entire cellulosome and a detailed picture of intermodular contacts will provide more detailed insight into cellulosome activity. Toward this goal, we have solved the structure of a multimodular heterodimeric complex from Clostridium thermocellum composed of the type-II cohesin module of the cell surface protein SdbA bound to a trimodular C-terminal fragment of the scaffoldin subunit CipA to a resolution of 1.95 A. The linker that connects the ninth type-I cohesin module and the X module has elevated temperature factors, reflecting an inherent flexibility within this region. Interestingly, a novel dimer interface was observed between CipA and a second, symmetry-related CipA molecule within the crystal structure, mediated by contacts between a type-I cohesin and an X module of a symmetry mate, resulting in two intertwined scaffoldins. Sedimentation velocity experiments confirmed that dimerization also occurs in solution. These observations support the intriguing possibility that individual cellulosomes can associate with one another via inter-scaffoldin interactions, which may play a role in the mechanism of action of the complex.

PubMed: 20070943
DOI: 10.1016/j.jmb.2010.01.015

実験手法

X-RAY DIFFRACTION (1.94 Å)