3K7A

Crystal Structure of an RNA polymerase II-TFIIB complex

3K7A の概要

• エントリーDOI: 10.2210/pdb3k7a/pdb
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC4, Transcription initiation factor IIB, ... (12 entities in total)
• 機能のキーワード: rna polymerase ii, tfiib, transcription, dna-binding, dna-directed rna polymerase, isopeptide bond, magnesium, metal-binding, nucleotidyltransferase, nucleus, phosphoprotein, transferase, zinc-finger, dna damage, dna repair, transcription regulation
• 由来する生物種: Saccharomyces cerevisiae (yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P29055 P08518 P16370 P20434 P20436 P27999 P38902; Nucleus, nucleolus: P40422 P22139; Cytoplasm: P20435
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 508472.00

構造登録者

Liu, X.,Bushnell, D.A.,Wang, D.,Calero, G.,Kornberg, R.D. (登録日: 2009-10-12, 公開日: 2009-11-24, 最終更新日: 2024-11-27)

主引用文献

Liu, X.,Bushnell, D.A.,Wang, D.,Calero, G.,Kornberg, R.D.
Structure of an RNA polymerase II-TFIIB complex and the transcription initiation mechanism.
Science, 327:206-209, 2010

PubMed Abstract: Previous x-ray crystal structures have given insight into the mechanism of transcription and the role of general transcription factors in the initiation of the process. A structure of an RNA polymerase II-general transcription factor TFIIB complex at 4.5 angstrom resolution revealed the amino-terminal region of TFIIB, including a loop termed the "B finger," reaching into the active center of the polymerase where it may interact with both DNA and RNA, but this structure showed little of the carboxyl-terminal region. A new crystal structure of the same complex at 3.8 angstrom resolution obtained under different solution conditions is complementary with the previous one, revealing the carboxyl-terminal region of TFIIB, located above the polymerase active center cleft, but showing none of the B finger. In the new structure, the linker between the amino- and carboxyl-terminal regions can also be seen, snaking down from above the cleft toward the active center. The two structures, taken together with others previously obtained, dispel long-standing mysteries of the transcription initiation process.

PubMed: 19965383
DOI: 10.1126/science.1182015

実験手法

X-RAY DIFFRACTION (3.8 Å)