3K5G

Human bace-1 complex with bjc060

3K5G の概要

• エントリーDOI: 10.2210/pdb3k5g/pdb
• 関連するPDBエントリー: 3K5C 3K5D 3K5F
• 分子名称: Beta-secretase 1, (1R,3S)-N-[(1S,2R)-1-benzyl-2-hydroxy-3-{[3-(1-methylethyl)benzyl]amino}propyl]-3-[1-methyl-1-(2-oxopiperidin-1-yl)ethy l]cyclohexanecarboxamide (3 entities in total)
• 機能のキーワード: aspartyl protease, alzheimer's disease, structure-based design, disulfide bond, endoplasmic reticulum, endosome, glycoprotein, golgi apparatus, hydrolase-hydrolase inhibitor complex, membrane, protease, transmembrane, hydrolase/hydrolase inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P56817
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 136017.40

構造登録者

Rondeau, J.-M. (登録日: 2009-10-07, 公開日: 2010-05-05, 最終更新日: 2024-11-20)

主引用文献

Hanessian, S.,Shao, Z.,Betschart, C.,Rondeau, J.M.,Neumann, U.,Tintelnot-Blomley, M.
Structure-based design and synthesis of novel P2/P3 modified, non-peptidic beta-secretase (BACE-1) inhibitors.
Bioorg.Med.Chem.Lett., 20:1924-1927, 2010

PubMed Abstract: Starting from peptidomimetic BACE-1 inhibitors, the P2 amino acid including the P2/P3 peptide bond was replaced by a rigid 3-aminomethyl cyclohexane carboxylic acid. Co-crystallization revealed an unexpected binding mode with the P3/P4 amide bond placed into the S3 pocket resulting in a new hydrogen bond interaction pattern. Further optimization based on this structure resulted in highly potent BACE-1 inhibitors with selectivity over BACE-2 and cathepsin D.

PubMed: 20172717
DOI: 10.1016/j.bmcl.2010.01.139

実験手法

X-RAY DIFFRACTION (2 Å)