3K4P

Aspergillus niger Phytase

3K4P の概要

• エントリーDOI: 10.2210/pdb3k4p/pdb
• 関連するPDBエントリー: 1dkl 1dkq 1ihp 1qfx 2gfi 3k4q
• 分子名称: 3-phytase A, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: phytase, phya, 3-phosphotase, myo-inositol hexakis phosphate phosphohydrolase, 37288-11-2, disulfide bond, glycoprotein, hydrolase, secreted
• 由来する生物種: Aspergillus niger
• 細胞内の位置: Secreted: P34752
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99105.24

構造登録者

Oakley, A.J. (登録日: 2009-10-06, 公開日: 2010-06-30, 最終更新日: 2024-10-30)

主引用文献

Oakley, A.J.
The structure of Aspergillus niger phytase PhyA in complex with a phytate mimetic
Biochem.Biophys.Res.Commun., 397:745-749, 2010

PubMed Abstract: Phytases hydrolyse the phosphomonoesters of phytate (myo-inositol-1,2,3,4,5,6-hexakis phosphate) and thus find uses in plant and animal production through the mobilisation of phosphorus from this source. The structure of partially deglycosylated Aspergillus niger PhyA is presented in apo form and in complex with the potent inhibitor myo-inositol-1,2,3,4,5,6-hexakis sulfate, which by analogy with phytate provides a snapshot of the Michaelis complex. The structure explains the enzyme's preference for the 3'-phosphate of phytate. The apo-and inhibitor-bound forms are similar and no induced-fit mechanism operates. Furthermore the enzyme structure is apparently unaffected by the presence of glycosides on the surface. The new structures of A. niger PhyA are discussed in the context of protein engineering studies aimed at modulating pH preference and stability.

PubMed: 20541524
DOI: 10.1016/j.bbrc.2010.06.024

実験手法

X-RAY DIFFRACTION (2.4 Å)