3K4E

Puf3 RNA binding domain bound to Cox17 RNA 3' UTR recognition sequence site A

3K4E の概要

• エントリーDOI: 10.2210/pdb3k4e/pdb
• 関連するPDBエントリー: 3k49
• 分子名称: mRNA-binding protein PUF3, RNA (5'-R(P*CP*UP*UP*GP*UP*AP*UP*AP*UP*A)-3') (3 entities in total)
• 機能のキーワード: puf3, pumilio, rna binding, mitochondrial mrna, membrane, mitochondrion, mitochondrion outer membrane, phosphoprotein, rna-binding, rna binding protein - rna complex, rna binding protein / rna
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast); 詳細
• 細胞内の位置: Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side: Q07807
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 136376.99

構造登録者

Zhu, D.,Stumpf, C.R.,Krahn, J.M.,Wickens, M.,Hall, T.M.T. (登録日: 2009-10-05, 公開日: 2009-10-27, 最終更新日: 2023-09-06)

主引用文献

Zhu, D.,Stumpf, C.R.,Krahn, J.M.,Wickens, M.,Hall, T.M.
A 5' cytosine binding pocket in Puf3p specifies regulation of mitochondrial mRNAs.
Proc.Natl.Acad.Sci.USA, 106:20192-20197, 2009

PubMed Abstract: A single regulatory protein can control the fate of many mRNAs with related functions. The Puf3 protein of Saccharomyces cerevisiae is exemplary, as it binds and regulates more than 100 mRNAs that encode proteins with mitochondrial function. Here we elucidate the structural basis of that specificity. To do so, we explore the crystal structures of Puf3p complexes with 2 cognate RNAs. The key determinant of Puf3p specificity is an unusual interaction between a distinctive pocket of the protein with an RNA base outside the "core" PUF-binding site. That interaction dramatically affects binding affinity in vitro and is required for regulation in vivo. The Puf3p structures, combined with those of Puf4p in the same organism, illuminate the structural basis of natural PUF-RNA networks. Yeast Puf3p binds its own RNAs because they possess a -2C and is excluded from those of Puf4p which contain an additional nucleotide in the core-binding site.

PubMed: 19918084
DOI: 10.1073/pnas.0812079106

実験手法

X-RAY DIFFRACTION (3.2 Å)