3K30

Histamine dehydrogenase from Nocardiodes simplex

3K30 の概要

• エントリーDOI: 10.2210/pdb3k30/pdb
• 分子名称: Histamine dehydrogenase, IRON/SULFUR CLUSTER, FLAVIN MONONUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: 6-s-cysteinyl-fmn, adp binding site, oxidoreductase
• 由来する生物種: Pimelobacter simplex (Arthrobacter simplex)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 153783.03

構造登録者

Scott, E.E.,Reed, T.M.,Limburg, J. (登録日: 2009-09-30, 公開日: 2010-05-26, 最終更新日: 2023-09-06)

主引用文献

Reed, T.,Lushington, G.H.,Xia, Y.,Hirakawa, H.,Travis, D.M.,Mure, M.,Scott, E.E.,Limburg, J.
Crystal structure of histamine dehydrogenase from Nocardioides simplex.
J.Biol.Chem., 285:25782-25791, 2010

PubMed Abstract: Histamine dehydrogenase (HADH) isolated from Nocardioides simplex catalyzes the oxidative deamination of histamine to imidazole acetaldehyde. HADH is highly specific for histamine, and we are interested in understanding the recognition mode of histamine in its active site. We describe the first crystal structure of a recombinant form of HADH (HADH) to 2.7-A resolution. HADH is a homodimer, where each 76-kDa subunit contains an iron-sulfur cluster ([4Fe-4S](2+)) and a 6-S-cysteinyl flavin mononucleotide (6-S-Cys-FMN) as redox cofactors. The overall structure of HADH is very similar to that of trimethylamine dehydrogenase (TMADH) from Methylotrophus methylophilus (bacterium W3A1). However, some distinct differences between the structure of HADH and TMADH have been found. Tyr(60), Trp(264), and Trp(355) provide the framework for the "aromatic bowl" that serves as a trimethylamine-binding site in TMADH is comprised of Gln(65), Trp(267), and Asp(358), respectively, in HADH. The surface Tyr(442) that is essential in transferring electrons to electron-transfer flavoprotein (ETF) in TMADH is not conserved in HADH. We use this structure to propose the binding mode for histamine in the active site of HADH through molecular modeling and to compare the interactions to those observed for other histamine-binding proteins whose structures are known.

PubMed: 20538584
DOI: 10.1074/jbc.M109.084301

実験手法

X-RAY DIFFRACTION (2.7 Å)