3K2B

Crystal structure of photosynthetic A4 isoform glyceraldehyde-3-phosphate dehydrogenase complexed with NAD, from Arabidopsis thaliana

Summary for 3K2B

• Entry DOI: 10.2210/pdb3k2b/pdb
• Related: 1nbo
• Descriptor: Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, SULFATE ION, ... (4 entities in total)
• Functional Keywords: rossmann fold, calvin cycle, chloroplast, membrane, nadp, oxidoreductase, transit peptide
• Biological source: Arabidopsis thaliana (thale cress)
• Cellular location: Plastid, chloroplast membrane; Peripheral membrane protein: P25856
• Total number of polymer chains: 10
• Total formula weight: 374872.79

Authors

Fermani, S.,Falini, G.,Thumiger, A.,Sparla, F.,Marri, L.,Trost, P. (deposition date: 2009-09-29, release date: 2010-06-09, Last modification date: 2023-11-01)

Primary citation

Fermani, S.,Sparla, F.,Marri, L.,Thumiger, A.,Pupillo, P.,Falini, G.,Trost, P.
Structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase (isoform A4) from Arabidopsis thaliana in complex with NAD
Acta Crystallogr.,Sect.F, 66:621-626, 2010

PubMed Abstract: The crystal structure of the A(4) isoform of photosynthetic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Arabidopsis thaliana, expressed in recombinant form and complexed with NAD, is reported. The crystals, which were grown in 2.4 M ammonium sulfate and 0.1 M sodium citrate, belonged to space group I222. The asymmetric unit includes ten subunits, i.e. two independent tetramers plus a dimer that generates a third tetramer by a crystallographic symmetry operation. The crystal structure was solved by molecular replacement and refined to an R factor of 23.7% and an R(free) factor of 28.9% at 2.6 A resolution. In the final model, each subunit binds one NAD(+) molecule and two sulfates, which occupy the P(s) and the P(i) anion-binding sites. Detailed knowledge of this structure is instrumental for structural investigation of supramolecular complexes of A(4)-GAPDH, phosphoribulokinase and CP12, which are involved in the regulation of photosynthesis in the model plant A. thaliana.

PubMed: 20516587
DOI: 10.1107/S1744309110013527

Experimental method

X-RAY DIFFRACTION (2.6 Å)