3K0T

Crystal structure of PSPTO -PSP protein in complex with D-beta-Glucose from Pseudomonas syringae pv. tomato str. DC3000

3K0T の概要

• エントリーDOI: 10.2210/pdb3k0t/pdb
• 分子名称: Endoribonuclease L-PSP, putative, beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: psp, glucose binding, er stressor, sugar binding protein
• 由来する生物種: Pseudomonas syringae pv. tomato
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 47646.28

構造登録者

Zhang, H.M.,Li, M.,Chang, W.R. (登録日: 2009-09-25, 公開日: 2010-06-16, 最終更新日: 2024-11-06)

主引用文献

Zhang, H.M.,Gao, Y.,Li, M.,Chang, W.R.
Crystal structure of the PSPTO-PSP protein from Pseudomonas syringae pv. tomato str. DC3000 in complex with D-glucose
Biochem.Biophys.Res.Commun., 397:82-86, 2010

PubMed Abstract: The perchloric acid-soluble protein (PSP) is an endoribonuclease and on the basis of sequence similarity has been assigned to the YjgF/YER057c/UK114 family. These family members are ubiquitous and highly conserved in evolution, and participate in regulating basic cellular metabolism. Here we present the 2.1A crystal structure of the PSP protein from Pseudomonas syringae pv. tomato str. DC3000 (PSPTO-PSP), in complex with D-glucose. The quaternary structure of PSPTO-PSP is a homologous trimer. Glucose is located in the cavity between each two monomers. Comparison of the hydrogen bonds between ligands and YjgF/YER057c/UK114 family homologues confirms that the conserved Arg(103) of PSPTO-PSP is a key amino acid in this cavity for ligand binding. It indicated that the involvement of PSPTO-PSP in essential cellular mechanism was regulated by glucose occupying this active site.

PubMed: 20478270
DOI: 10.1016/j.bbrc.2010.05.071

実験手法

X-RAY DIFFRACTION (2.1 Å)