3JYO

Quinate dehydrogenase from Corynebacterium glutamicum in complex with NAD

3JYO の概要

• エントリーDOI: 10.2210/pdb3jyo/pdb
• 関連するPDBエントリー: 2nlo 3JYP 3JYQ
• 分子名称: Quinate/shikimate dehydrogenase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: enzyme-cofactor complex, amino-acid biosynthesis, aromatic amino acid biosynthesis, nad, oxidoreductase
• 由来する生物種: Corynebacterium glutamicum (Brevibacterium flavum)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30388.01

構造登録者

Hoeppner, A.,Niefind, K.,Schomburg, D. (登録日: 2009-09-22, 公開日: 2010-10-27, 最終更新日: 2023-09-06)

主引用文献

Hoppner, A.,Schomburg, D.,Niefind, K.
Enzyme-substrate complexes of the quinate/shikimate dehydrogenase from Corynebacterium glutamicum enable new insights in substrate and cofactor binding, specificity, and discrimination.
Biol.Chem., 394:1505-1516, 2013

PubMed Abstract: Quinate dehydrogenase (QDH) catalyzes the reversible oxidation of quinate to 3-dehydroquinate by nicotineamide adenine dinucleotide (NADH) and is involved in the catabolic quinate metabolism required for the degradation of lignin. The enzyme is a member of the family of shikimate/quinate dehydrogenases (SDH/QDH) occurring in bacteria and plants. We characterized the dual-substrate quinate/shikimate dehydrogenase (QSDH) from Corynebacterium glutamicum (CglQSDH) kinetically and revealed a clear substrate preference of CglQSDH for quinate compared with shikimate both at the pH optimum and in a physiological pH range, which is a remarkable contrast to closely related SDH/QDH enzymes. With respect to the cosubstrate, CglQSDH is strictly NAD(H) dependent. These substrate and cosubstrate profiles correlate well with the details of three atomic resolution crystal structures of CglQSDH in different functional states we report here: with bound NAD+ (binary complex) and as ternary complexes with NADH plus either shikimate or quinate. The CglQSDH-NADH-quinate structure is the first complex structure of any member of the SDH/QDH family with quinate. Based on this novel structural information and systematic sequence and structure comparisons with closely related enzymes, we can explain the strict NAD(H) dependency of CglQSDH as well as its discrimination between shikimate and quinate.

PubMed: 23929881
DOI: 10.1515/hsz-2013-0170

実験手法

X-RAY DIFFRACTION (1 Å)