3JYC

Crystal structure of the eukaryotic strong inward-rectifier K+ channel Kir2.2 at 3.1 Angstrom resolution

3JYC の概要

• エントリーDOI: 10.2210/pdb3jyc/pdb
• 分子名称: Inward-rectifier K+ channel Kir2.2, POTASSIUM ION (2 entities in total)
• 機能のキーワード: a transmembrane pore and a cytoplasmic pore, metal transport
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39557.21

構造登録者

Tao, X. (登録日: 2009-09-21, 公開日: 2010-01-12, 最終更新日: 2024-10-30)

主引用文献

Tao, X.,Avalos, J.L.,Chen, J.,MacKinnon, R.
Crystal structure of the eukaryotic strong inward-rectifier K+ channel Kir2.2 at 3.1 A resolution.
Science, 326:1668-1674, 2009

PubMed Abstract: Inward-rectifier potassium (K+) channels conduct K+ ions most efficiently in one direction, into the cell. Kir2 channels control the resting membrane voltage in many electrically excitable cells, and heritable mutations cause periodic paralysis and cardiac arrhythmia. We present the crystal structure of Kir2.2 from chicken, which, excluding the unstructured amino and carboxyl termini, is 90% identical to human Kir2.2. Crystals containing rubidium (Rb+), strontium (Sr2+), and europium (Eu3+) reveal binding sites along the ion conduction pathway that are both conductive and inhibitory. The sites correlate with extensive electrophysiological data and provide a structural basis for understanding rectification. The channel's extracellular surface, with large structured turrets and an unusual selectivity filter entryway, might explain the relative insensitivity of eukaryotic inward rectifiers to toxins. These same surface features also suggest a possible approach to the development of inhibitory agents specific to each member of the inward-rectifier K+ channel family.

PubMed: 20019282
DOI: 10.1126/science.1180310

実験手法

X-RAY DIFFRACTION (3.11 Å)