3JX7

Bacillus cereus alkylpurine DNA glycosylase AlkD bound to DNA containing a 3-METHYLADENINE analog

3JX7 の概要

• エントリーDOI: 10.2210/pdb3jx7/pdb
• 関連するPDBエントリー: 3BVS 3JXY 3JXZ 3JY1
• 分子名称: alkylpurine DNA glycosylase AlkD, DNA (5'-D(*CP*GP*GP*AP*CP*TP*(DZM)P*AP*CP*GP*GP*G)-3'), DNA (5'-D(*CP*CP*CP*GP*TP*TP*AP*GP*TP*CP*CP*G)-3'), ... (4 entities in total)
• 機能のキーワード: heat repeat, dna binding, dna glycosylase, dna alkylation, lyase-dna complex, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Bacillus cereus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 35063.90

構造登録者

Rubinson, E.H.,Eichman, B.F. (登録日: 2009-09-18, 公開日: 2010-09-22, 最終更新日: 2023-09-06)

主引用文献

Rubinson, E.H.,Gowda, A.S.,Spratt, T.E.,Gold, B.,Eichman, B.F.
An unprecedented nucleic acid capture mechanism for excision of DNA damage.
Nature, 468:406-411, 2010

PubMed Abstract: DNA glycosylases that remove alkylated and deaminated purine nucleobases are essential DNA repair enzymes that protect the genome, and at the same time confound cancer alkylation therapy, by excising cytotoxic N3-methyladenine bases formed by DNA-targeting anticancer compounds. The basis for glycosylase specificity towards N3- and N7-alkylpurines is believed to result from intrinsic instability of the modified bases and not from direct enzyme functional group chemistry. Here we present crystal structures of the recently discovered Bacillus cereus AlkD glycosylase in complex with DNAs containing alkylated, mismatched and abasic nucleotides. Unlike other glycosylases, AlkD captures the extrahelical lesion in a solvent-exposed orientation, providing an illustration for how hydrolysis of N3- and N7-alkylated bases may be facilitated by increased lifetime out of the DNA helix. The structures and supporting biochemical analysis of base flipping and catalysis reveal how the HEAT repeats of AlkD distort the DNA backbone to detect non-Watson-Crick base pairs without duplex intercalation.

PubMed: 20927102
DOI: 10.1038/nature09428

実験手法

X-RAY DIFFRACTION (1.6 Å)