3JUU

Crystal structure of porphyranase B (PorB) from Zobellia galactanivorans

3JUU の概要

• エントリーDOI: 10.2210/pdb3juu/pdb
• 関連するPDBエントリー: 3ILF
• 分子名称: porphyranase B, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: glycoside hydrolase family gh16, beta-sandwich fold, jelly roll, sugar binding protein, hydrolase-carbohydrate complex, hydrolase/carbohydrate
• 由来する生物種: Zobellia galactanivorans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66091.24

構造登録者

Hehemann, J.H.,Correc, G.,Barbeyron, T.,Michel, G.,Czjzek, M. (登録日: 2009-09-15, 公開日: 2010-04-07, 最終更新日: 2024-10-09)

主引用文献

Hehemann, J.H.,Correc, G.,Barbeyron, T.,Helbert, W.,Czjzek, M.,Michel, G.
Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota.
Nature, 464:908-912, 2010

PubMed Abstract: Gut microbes supply the human body with energy from dietary polysaccharides through carbohydrate active enzymes, or CAZymes, which are absent in the human genome. These enzymes target polysaccharides from terrestrial plants that dominated diet throughout human evolution. The array of CAZymes in gut microbes is highly diverse, exemplified by the human gut symbiont Bacteroides thetaiotaomicron, which contains 261 glycoside hydrolases and polysaccharide lyases, as well as 208 homologues of susC and susD-genes coding for two outer membrane proteins involved in starch utilization. A fundamental question that, to our knowledge, has yet to be addressed is how this diversity evolved by acquiring new genes from microbes living outside the gut. Here we characterize the first porphyranases from a member of the marine Bacteroidetes, Zobellia galactanivorans, active on the sulphated polysaccharide porphyran from marine red algae of the genus Porphyra. Furthermore, we show that genes coding for these porphyranases, agarases and associated proteins have been transferred to the gut bacterium Bacteroides plebeius isolated from Japanese individuals. Our comparative gut metagenome analyses show that porphyranases and agarases are frequent in the Japanese population and that they are absent in metagenome data from North American individuals. Seaweeds make an important contribution to the daily diet in Japan (14.2 g per person per day), and Porphyra spp. (nori) is the most important nutritional seaweed, traditionally used to prepare sushi. This indicates that seaweeds with associated marine bacteria may have been the route by which these novel CAZymes were acquired in human gut bacteria, and that contact with non-sterile food may be a general factor in CAZyme diversity in human gut microbes.

PubMed: 20376150
DOI: 10.1038/nature08937

実験手法

X-RAY DIFFRACTION (1.8 Å)