3JU9

Crystal structure of a lectin from Canavalia brasiliensis seed (ConBr) complexed with alpha-aminobutyric acid

Summary for 3JU9

• Entry DOI: 10.2210/pdb3ju9/pdb
• Related: 1azd
• Descriptor: Concanavalin-Br, D-ALPHA-AMINOBUTYRIC ACID, GLYCEROL, ... (7 entities in total)
• Functional Keywords: conbr, lectin, agglutinin, canavalia brasiliensis, manganese, metal-binding, sugar binding protein
• Biological source: Canavalia brasiliensis (Brazilian jack bean)
• Total number of polymer chains: 1
• Total formula weight: 25953.56

Authors

Bezerra, E.H.S.,Rocha, B.A.M.,Nagano, C.S.,Bezerra, G.A.,Moura, T.R.,Bezerra, M.J.B.,Benevides, R.G.,Marinho, E.S.,Delatorre, P.,Cavada, B.S. (deposition date: 2009-09-14, release date: 2010-12-08, Last modification date: 2023-11-22)

Primary citation

Bezerra, E.H.,Rocha, B.A.,Nagano, C.S.,Bezerra, G.A.,Moura, T.R.,Bezerra, M.J.,Benevides, R.G.,Sampaio, A.H.,Assreuy, A.M.,Delatorre, P.,Cavada, B.S.
Structural analysis of ConBr reveals molecular correlation between the carbohydrate recognition domain and endothelial NO synthase activation.
Biochem.Biophys.Res.Commun., 408:566-570, 2011

PubMed Abstract: Diocleinae lectins are highly homologous in their primary structure which features metal binding sites and a carbohydrate recognition domain (CRD). Differences in the biological activity of legume lectins have been widely investigated using hemagglutination inhibition assays, isothermal titration microcalorimetry and co-crystallization with mono- and oligosaccharides. Here we report a new lectin crystal structure (ConBr) extracted from seeds of Canavalia brasiliensis, predict dimannoside binding by docking, identify the α-aminobutyric acid (Abu) binding pocket and compare the CRD of ConBr to that of homologous lectins. Based on the hypothesis that the carbohydrate affinity of lectins depends on CRD configuration, the relationship between tridimensional structure and endothelial NO synthase activation was used to clarify differences in biological activity. Our study established a correlation between the position of CRD amino acid side chains and the stimulation of NO release from endothelium.

PubMed: 21530490
DOI: 10.1016/j.bbrc.2011.04.061

Experimental method

X-RAY DIFFRACTION (2.1 Å)