3JTY

Crystal structure of a BenF-like porin from Pseudomonas fluorescens Pf-5

Summary for 3JTY

• Entry DOI: 10.2210/pdb3jty/pdb
• Descriptor: BenF-like porin, LAURYL DIMETHYLAMINE-N-OXIDE (3 entities in total)
• Functional Keywords: pfl_1329, benf-like porin, pseudomonas fluorescens pf-5, benzoate transporter, efflux pump, structural genomics, psi-2, protein structure initiative, new york structural genomix research consortium, nysgxrc, new york sgx research center for structural genomics, transport protein
• Biological source: Pseudomonas fluorescens Pf-5
• Total number of polymer chains: 4
• Total formula weight: 179416.90

Authors

Sampathkumar, P.,Lu, F.,Zhao, X.,Wasserman, S.,Iuzuka, M.,Bain, K.,Rutter, M.,Gheyi, T.,Atwell, S.,Luz, J.,Gilmore, J.,Sauder, J.M.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (deposition date: 2009-09-14, release date: 2009-10-20, Last modification date: 2023-09-06)

Primary citation

Sampathkumar, P.,Lu, F.,Zhao, X.,Li, Z.,Gilmore, J.,Bain, K.,Rutter, M.E.,Gheyi, T.,Schwinn, K.D.,Bonanno, J.B.,Pieper, U.,Fajardo, J.E.,Fiser, A.,Almo, S.C.,Swaminathan, S.,Chance, M.R.,Baker, D.,Atwell, S.,Thompson, D.A.,Emtage, J.S.,Wasserman, S.R.,Sali, A.,Sauder, J.M.,Burley, S.K.
Structure of a putative BenF-like porin from Pseudomonas fluorescens Pf-5 at 2.6 A resolution.
Proteins, 78:3056-3062, 2010

PubMed Abstract: The X-ray structure of a putative BenF-like (gene name: PFL1329) protein from (PflBenF) has been determined at 2.6Å resolution. X-ray crystallography revealed a canonical 18-stranded β-barrel fold that forms a central pore with a diameter of ∼4.6Å, which is consistent with the size and physicochemical properties of the presumed aromatic acid substrate, benzoate. Detailed comparisons with the previously-determined structure of OpdK, a vanillate influx channel, revealed an arginine-rich aromatic acid selectivity filter of nearly identical structure composed of seven highly conserved residues Arg∼Asp∼Arg∼Arg∼Ser∼Asp∼Arg (R∼D∼R∼R∼S∼D∼R sequence motif, where ∼ denotes intervening residues) that define the narrowest part of the pore.

PubMed: 20737437
DOI: 10.1002/prot.22829

Experimental method

X-RAY DIFFRACTION (2.58 Å)