3JSC

CcdBVfi-FormI-pH7.0

Summary for 3JSC

• Entry DOI: 10.2210/pdb3jsc/pdb
• Related: 1VUB 2VUB 3JRZ 3VUB 4VUB
• Descriptor: CcdB, SULFATE ION (3 entities in total)
• Functional Keywords: alpha+beta, sh3 domain, toxin
• Biological source: Vibrio fischeri
• Total number of polymer chains: 1
• Total formula weight: 11975.78

Authors

De Jonge, N.,Buts, L.,Loris, R. (deposition date: 2009-09-10, release date: 2009-12-01, Last modification date: 2023-11-01)

Primary citation

De Jonge, N.,Hohlweg, W.,Garcia-Pino, A.,Respondek, M.,Buts, L.,Haesaerts, S.,Lah, J.,Zangger, K.,Loris, R.
Structural and thermodynamic characterization of vibrio fischeri CCDB
J.Biol.Chem., 285:5606-5613, 2010

PubMed Abstract: CcdB(Vfi) from Vibrio fischeri is a member of the CcdB family of toxins that poison covalent gyrase-DNA complexes. In solution CcdB(Vfi) is a dimer that unfolds to the corresponding monomeric components in a two-state fashion. In the unfolded state, the monomer retains a partial secondary structure. This observation correlates well with the crystal and NMR structures of the protein, which show a dimer with a hydrophobic core crossing the dimer interface. In contrast to its F plasmid homologue, CcdB(Vfi) possesses a rigid dimer interface, and the apparent relative rotations of the two subunits are due to structural plasticity of the monomer. CcdB(Vfi) shows a number of non-conservative substitutions compared with the F plasmid protein in both the CcdA and the gyrase binding sites. Although variation in the CcdA interaction site likely determines toxin-antitoxin specificity, substitutions in the gyrase-interacting region may have more profound functional implications.

PubMed: 19959472
DOI: 10.1074/jbc.M109.068429

Experimental method

X-RAY DIFFRACTION (1.5 Å)