3JRQ

Crystal structure of (+)-ABA-bound PYL1 in complex with ABI1

3JRQ の概要

• エントリーDOI: 10.2210/pdb3jrq/pdb
• 関連するPDBエントリー: 3JRS
• 分子名称: Protein phosphatase 2C 56, Putative uncharacterized protein At5g46790, (2Z,4E)-5-[(1S)-1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-1-yl]-3-methylpenta-2,4-dienoic acid, ... (4 entities in total)
• 機能のキーワード: plant hormone receptor, abscisic acid, pyl1, abi1, type 2c protein phosphatase, abscisic acid signaling pathway, cell membrane, hydrolase, magnesium, manganese, membrane, metal-binding, nucleus, protein phosphatase, hydrolase-hormone receptor complex, hydrolase/hormone receptor
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• 細胞内の位置: Nucleus: P49597; Cytoplasm (By similarity): Q8VZS8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57587.24

構造登録者

Miyazono, K.,Miyakawa, T.,Sawano, Y.,Kubota, K.,Tanokura, M. (登録日: 2009-09-08, 公開日: 2009-11-03, 最終更新日: 2023-11-01)

主引用文献

Miyazono, K.,Miyakawa, T.,Sawano, Y.,Kubota, K.,Kang, H.J.,Asano, A.,Miyauchi, Y.,Takahashi, M.,Zhi, Y.,Fujita, Y.,Yoshida, T.,Kodaira, K.,Yamaguchi-Shinozaki, K.,Tanokura, M.
Structural basis of abscisic acid signalling
Nature, 462:609-614, 2009

PubMed Abstract: The phytohormone abscisic acid (ABA) mediates the adaptation of plants to environmental stresses such as drought and regulates developmental signals such as seed maturation. Within plants, the PYR/PYL/RCAR family of START proteins receives ABA to inhibit the phosphatase activity of the group-A protein phosphatases 2C (PP2Cs), which are major negative regulators in ABA signalling. Here we present the crystal structures of the ABA receptor PYL1 bound with (+)-ABA, and the complex formed by the further binding of (+)-ABA-bound PYL1 with the PP2C protein ABI1. PYL1 binds (+)-ABA using the START-protein-specific ligand-binding site, thereby forming a hydrophobic pocket on the surface of the closed lid. (+)-ABA-bound PYL1 tightly interacts with a PP2C domain of ABI1 by using the hydrophobic pocket to cover the active site of ABI1 like a plug. Our results reveal the structural basis of the mechanism of (+)-ABA-dependent inhibition of ABI1 by PYL1 in ABA signalling.

PubMed: 19855379
DOI: 10.1038/nature08583

実験手法

X-RAY DIFFRACTION (2.1 Å)