3JRO

NUP84-NUP145C-SEC13 edge element of the NPC lattice

3JRO の概要

• エントリーDOI: 10.2210/pdb3jro/pdb
• 関連するPDBエントリー: 3JRP
• 分子名称: Fusion Protein of Protein Transport Protein SEC13 and Nucleoporin NUP145, Nucleoporin NUP84 (2 entities in total)
• 機能のキーワード: protein complex, cytoplasmic vesicle, endoplasmic reticulum, er-golgi transport, membrane, mrna transport, nuclear pore complex, nucleus, protein transport, translocation, transport, wd repeat, autocatalytic cleavage, hydrolase, phosphoprotein, transport protein, structural protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleoporin NUP145C: Nucleus, nuclear pore complex. Nucleoporin NUP145N: Nucleus, nuclear pore complex: P49687; Nucleus, nuclear pore complex: P52891
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 134612.63

構造登録者

Brohawn, S.G.,Schwartz, T.U. (登録日: 2009-09-08, 公開日: 2009-10-27, 最終更新日: 2024-11-20)

主引用文献

Brohawn, S.G.,Schwartz, T.U.
Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice.
Nat.Struct.Mol.Biol., 16:1173-1177, 2009

PubMed Abstract: Nuclear pore complexes (NPCs) facilitate all nucleocytoplasmic transport. These massive protein assemblies are modular, with a stable structural scaffold supporting more dynamically attached components. The scaffold is made from multiple copies of the heptameric Y complex and the heteromeric Nic96 complex. We previously showed that members of these core subcomplexes specifically share an ACE1 fold with Sec31 of the COPII vesicle coat, and we proposed a lattice model for the NPC based on this commonality. Here we present the crystal structure of the heterotrimeric 134-kDa complex of Nup84-Nup145C-Sec13 of the Y complex. The heterotypic ACE1 interaction of Nup84 and Nup145C is analogous to the homotypic ACE1 interaction of Sec31 that forms COPII lattice edge elements and is inconsistent with the alternative 'fence-like' NPC model. We construct a molecular model of the Y complex and compare the architectural principles of COPII and NPC lattices.

PubMed: 19855394
DOI: 10.1038/nsmb.1713

実験手法

X-RAY DIFFRACTION (4.004 Å)