3JC9

Architectural model of the type IVa pilus machine in a non-piliated state

これはPDB形式変換不可エントリーです。

3JC9 の概要

• エントリーDOI: 10.2210/pdb3jc9/pdb
• 関連するPDBエントリー: 3JC8
• EMDBエントリー: 3247 3260
• 分子名称: PilA, ADENOSINE-5'-TRIPHOSPHATE, PilC, ... (10 entities in total)
• 機能のキーワード: motor, pilus, ring, membrane channel, motor protein
• 由来する生物種: Myxococcus xanthus DK 1622; 詳細
• タンパク質・核酸の鎖数: 79
• 化学式量合計: 3172336.30

構造登録者

Chang, Y.-W.,Rettberg, L.A.,Jensen, G.J. (登録日: 2015-11-24, 公開日: 2016-03-16, 最終更新日: 2024-11-20)

主引用文献

Chang, Y.W.,Rettberg, L.A.,Treuner-Lange, A.,Iwasa, J.,Sgaard-Andersen, L.,Jensen, G.J.
Architecture of the type IVa pilus machine.
Science, 351:aad2001-aad2001, 2016

PubMed Abstract: Type IVa pili are filamentous cell surface structures observed in many bacteria. They pull cells forward by extending, adhering to surfaces, and then retracting. We used cryo-electron tomography of intact Myxococcus xanthus cells to visualize type IVa pili and the protein machine that assembles and retracts them (the type IVa pilus machine, or T4PM) in situ, in both the piliated and nonpiliated states, at a resolution of 3 to 4 nanometers. We found that T4PM comprises an outer membrane pore, four interconnected ring structures in the periplasm and cytoplasm, a cytoplasmic disc and dome, and a periplasmic stem. By systematically imaging mutants lacking defined T4PM proteins or with individual proteins fused to tags, we mapped the locations of all 10 T4PM core components and the minor pilins, thereby providing insights into pilus assembly, structure, and function.

PubMed: 26965631
DOI: 10.1126/science.aad2001

実験手法

ELECTRON MICROSCOPY