3J9X
A Virus that Infects a Hyperthermophile Encapsidates A-Form DNA
Summary for 3J9X
| Entry DOI | 10.2210/pdb3j9x/pdb |
| EMDB information | 6310 |
| Descriptor | coat protein, DNA (2 entities in total) |
| Functional Keywords | archaeal virus, helical symmetry, virus |
| Biological source | Sulfolobus islandicus rod-shaped virus 2 More |
| Total number of polymer chains | 60 |
| Total formula weight | 1012872.03 |
| Authors | DiMaio, F.,Yu, X.,Rensen, E.,Krupovic, M.,Prangishvili, D.,Egelman, E. (deposition date: 2015-03-21, release date: 2015-06-03, Last modification date: 2024-02-21) |
| Primary citation | DiMaio, F.,Yu, X.,Rensen, E.,Krupovic, M.,Prangishvili, D.,Egelman, E.H. A virus that infects a hyperthermophile encapsidates A-form DNA. Science, 348:914-917, 2015 Cited by PubMed Abstract: Extremophiles, microorganisms thriving in extreme environmental conditions, must have proteins and nucleic acids that are stable at extremes of temperature and pH. The nonenveloped, rod-shaped virus SIRV2 (Sulfolobus islandicus rod-shaped virus 2) infects the hyperthermophilic acidophile Sulfolobus islandicus, which lives at 80°C and pH 3. We have used cryo-electron microscopy to generate a three-dimensional reconstruction of the SIRV2 virion at ~4 angstrom resolution, which revealed a previously unknown form of virion organization. Although almost half of the capsid protein is unstructured in solution, this unstructured region folds in the virion into a single extended α helix that wraps around the DNA. The DNA is entirely in the A-form, which suggests a common mechanism with bacterial spores for protecting DNA in the most adverse environments. PubMed: 25999507DOI: 10.1126/science.aaa4181 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.8 Å) |
Structure validation
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