3J9X

A Virus that Infects a Hyperthermophile Encapsidates A-Form DNA

3J9X の概要

• エントリーDOI: 10.2210/pdb3j9x/pdb
• EMDBエントリー: 6310
• 分子名称: coat protein, DNA (2 entities in total)
• 機能のキーワード: archaeal virus, helical symmetry, virus
• 由来する生物種: Sulfolobus islandicus rod-shaped virus 2; 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 1012872.03

構造登録者

DiMaio, F.,Yu, X.,Rensen, E.,Krupovic, M.,Prangishvili, D.,Egelman, E. (登録日: 2015-03-21, 公開日: 2015-06-03, 最終更新日: 2024-02-21)

主引用文献

DiMaio, F.,Yu, X.,Rensen, E.,Krupovic, M.,Prangishvili, D.,Egelman, E.H.
A virus that infects a hyperthermophile encapsidates A-form DNA.
Science, 348:914-917, 2015

PubMed Abstract: Extremophiles, microorganisms thriving in extreme environmental conditions, must have proteins and nucleic acids that are stable at extremes of temperature and pH. The nonenveloped, rod-shaped virus SIRV2 (Sulfolobus islandicus rod-shaped virus 2) infects the hyperthermophilic acidophile Sulfolobus islandicus, which lives at 80°C and pH 3. We have used cryo-electron microscopy to generate a three-dimensional reconstruction of the SIRV2 virion at ~4 angstrom resolution, which revealed a previously unknown form of virion organization. Although almost half of the capsid protein is unstructured in solution, this unstructured region folds in the virion into a single extended α helix that wraps around the DNA. The DNA is entirely in the A-form, which suggests a common mechanism with bacterial spores for protecting DNA in the most adverse environments.

PubMed: 25999507
DOI: 10.1126/science.aaa4181

実験手法

ELECTRON MICROSCOPY (3.8 Å)