3J8D

Cryoelectron microscopy of dengue-Fab E104 complex at pH 5.5

3J8D の概要

• エントリーDOI: 10.2210/pdb3j8d/pdb
• EMDBエントリー: 6146
• 分子名称: glycoprotein DIII, antibody E111 Fab fragment, Envelope protein E (3 entities in total)
• 機能のキーワード: dengue virus, denv2 fab e104, low ph, fusion trimer, virus-immune system complex, virus/immune system
• 由来する生物種: Dengue virus 2 Thailand/16681/84 (DENV-2); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 245965.82

構造登録者

Zhang, X.Z.,Sheng, J.,Austin, S.K.,Hoornweg, T.,Smit, J.M.,Kuhn, R.J.,Diamond, M.S.,Rossmann, M.G. (登録日: 2014-10-13, 公開日: 2014-11-12, 最終更新日: 2024-02-21)

主引用文献

Zhang, X.,Sheng, J.,Austin, S.K.,Hoornweg, T.E.,Smit, J.M.,Kuhn, R.J.,Diamond, M.S.,Rossmann, M.G.
Structure of Acidic pH Dengue Virus Showing the Fusogenic Glycoprotein Trimers.
J.Virol., 89:743-750, 2015

PubMed Abstract: Flaviviruses undergo large conformational changes during their life cycle. Under acidic pH conditions, the mature virus forms transient fusogenic trimers of E glycoproteins that engage the lipid membrane in host cells to initiate viral fusion and nucleocapsid penetration into the cytoplasm. However, the dynamic nature of the fusogenic trimer has made the determination of its structure a challenge. Here we have used Fab fragments of the neutralizing antibody DV2-E104 to stop the conformational change of dengue virus at an intermediate stage of the fusion process. Using cryo-electron microscopy, we show that in this intermediate stage, the E glycoproteins form 60 trimers that are similar to the predicted "open" fusogenic trimer.

PubMed: 25355881
DOI: 10.1128/JVI.02411-14

実験手法

ELECTRON MICROSCOPY (26 Å)