3J8C

Model of the human eIF3 PCI-MPN octamer docked into the 43S EM map

「3J7K」から置き換えられました

3J8C の概要

• エントリーDOI: 10.2210/pdb3j8c/pdb
• 関連するPDBエントリー: 1RZ4 3CHM 3J47 3J8B 4B4T 4LCT 4O8X 4U1C 4U1D
• EMDBエントリー: 5658
• 分子名称: Eukaryotic translation initiation factor 3 subunit A, Eukaryotic translation initiation factor 3 subunit C, Eukaryotic translation initiation factor 3 subunit E, ... (8 entities in total)
• 機能のキーワード: translation
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 367531.88

構造登録者

Erzberger, J.P.,Ban, N. (登録日: 2014-10-08, 公開日: 2014-10-22, 最終更新日: 2024-02-21)

主引用文献

Erzberger, J.P.,Stengel, F.,Pellarin, R.,Zhang, S.,Schaefer, T.,Aylett, C.H.,Cimermancic, P.,Boehringer, D.,Sali, A.,Aebersold, R.,Ban, N.
Molecular Architecture of the 40SeIF1eIF3 Translation Initiation Complex.
Cell(Cambridge,Mass.), 158:1123-1135, 2014

PubMed Abstract: Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, six-subunit Saccharomyces cerevisiae eIF3 core. These structures, together with electron microscopy reconstructions, cross-linking coupled to mass spectrometry, and integrative structure modeling, allowed us to position and orient all eIF3 components on the 40S⋅eIF1 complex, revealing an extended, modular arrangement of eIF3 subunits. Yeast eIF3 engages 40S in a clamp-like manner, fully encircling 40S to position key initiation factors on opposite ends of the mRNA channel, providing a platform for the recruitment, assembly, and regulation of the translation initiation machinery. The structures of eIF3 components reported here also have implications for understanding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and the hepatitis C internal ribosomal entry site RNA.

PubMed: 25171412
DOI: 10.1016/j.cell.2014.07.044

実験手法

ELECTRON MICROSCOPY (11.6 Å)