3J7Y

Structure of the large ribosomal subunit from human mitochondria

3J7Y の概要

• エントリーDOI: 10.2210/pdb3j7y/pdb
• EMDBエントリー: 2762
• 分子名称: 16S rRNA, uL14, uL15, ... (54 entities in total)
• 機能のキーワード: mitochondria, large subunit, rrna, trna, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 51
• 化学式量合計: 1717077.64

構造登録者

Brown, A.,Amunts, A.,Bai, X.C.,Sugimoto, Y.,Edwards, P.C.,Murshudov, G.,Scheres, S.H.W.,Ramakrishnan, V. (登録日: 2014-08-26, 公開日: 2014-10-15, 最終更新日: 2024-05-29)

主引用文献

Brown, A.,Amunts, A.,Bai, X.C.,Sugimoto, Y.,Edwards, P.C.,Murshudov, G.,Scheres, S.H.,Ramakrishnan, V.
Structure of the large ribosomal subunit from human mitochondria.
Science, 346:718-722, 2014

PubMed Abstract: Human mitochondrial ribosomes are highly divergent from all other known ribosomes and are specialized to exclusively translate membrane proteins. They are linked with hereditary mitochondrial diseases and are often the unintended targets of various clinically useful antibiotics. Using single-particle cryogenic electron microscopy, we have determined the structure of its large subunit to 3.4 angstrom resolution, revealing 48 proteins, 21 of which are specific to mitochondria. The structure unveils an adaptation of the exit tunnel for hydrophobic nascent peptides, extensive remodeling of the central protuberance, including recruitment of mitochondrial valine transfer RNA (tRNA(Val)) to play an integral structural role, and changes in the tRNA binding sites related to the unusual characteristics of mitochondrial tRNAs.

PubMed: 25278503
DOI: 10.1126/science.1258026

実験手法

ELECTRON MICROSCOPY (3.4 Å)