3J7B

Catalase solved at 3.2 Angstrom resolution by MicroED

3J7B の概要

• エントリーDOI: 10.2210/pdb3j7b/pdb
• EMDBエントリー: 6314
• 分子名称: Catalase, PROTOPORPHYRIN IX CONTAINING FE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Bos taurus (bovine)
• 細胞内の位置: Peroxisome: P00432
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 245444.27

構造登録者

Nannenga, B.L.,Shi, D.,Hattne, J.,Reyes, F.E.,Gonen, T. (登録日: 2014-06-09, 公開日: 2014-10-08, 最終更新日: 2024-02-21)

主引用文献

Nannenga, B.L.,Shi, D.,Hattne, J.,Reyes, F.E.,Gonen, T.
Structure of catalase determined by MicroED.
Elife, 3:e03600-e03600, 2014

PubMed Abstract: MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 Å resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination.

PubMed: 25303172
DOI: 10.7554/eLife.03600

実験手法

ELECTRON CRYSTALLOGRAPHY (3.2 Å)