3J6K

2.5A structure of lysozyme solved by MicroED

Summary for 3J6K

• Entry DOI: 10.2210/pdb3j6k/pdb
• Related: 3J4G
• EMDB information: 6313
• Descriptor: Lysozyme C (2 entities in total)
• Functional Keywords: hydrolase
• Biological source: Gallus gallus (chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 14331.16

Authors

Nannenga, B.L.,Shi, D.,Leslie, A.G.W.,Gonen, T. (deposition date: 2014-03-18, release date: 2014-08-13, Last modification date: 2015-04-08)

Primary citation

Nannenga, B.L.,Shi, D.,Leslie, A.G.,Gonen, T.
High-resolution structure determination by continuous-rotation data collection in MicroED.
Nat.Methods, 11:927-930, 2014

PubMed Abstract: MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology.

PubMed: 25086503
DOI: 10.1038/nmeth.3043

Experimental method

ELECTRON CRYSTALLOGRAPHY (2.496 Å)