3J4G

Structure of lysozyme solved by MicroED to 2.9 A

3J4G の概要

• エントリーDOI: 10.2210/pdb3j4g/pdb
• EMDBエントリー: 2945
• 分子名称: Lysozyme C (2 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14331.16

構造登録者

Shi, D.,Nannenga, B.L.,Iadanza, M.G.,Gonen, T. (登録日: 2013-08-12, 公開日: 2013-11-13, 最終更新日: 2024-11-20)

主引用文献

Shi, D.,Nannenga, B.L.,Iadanza, M.G.,Gonen, T.
Three-dimensional electron crystallography of protein microcrystals.
Elife, 2:e01345-e01345, 2013

PubMed Abstract: We demonstrate that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). Lysozyme microcrystals were frozen on an electron microscopy grid, and electron diffraction data collected to 1.7 Å resolution. We developed a data collection protocol to collect a full-tilt series in electron diffraction to atomic resolution. A single tilt series contains up to 90 individual diffraction patterns collected from a single crystal with tilt angle increment of 0.1-1° and a total accumulated electron dose less than 10 electrons per angstrom squared. We indexed the data from three crystals and used them for structure determination of lysozyme by molecular replacement followed by crystallographic refinement to 2.9 Å resolution. This proof of principle paves the way for the implementation of a new technique, which we name 'MicroED', that may have wide applicability in structural biology. DOI: http://dx.doi.org/10.7554/eLife.01345.001.

PubMed: 24252878
DOI: 10.7554/eLife.01345

実験手法

ELECTRON CRYSTALLOGRAPHY (2.901 Å)