3J4A

Structure of gp8 connector protein

3J4A の概要

• エントリーDOI: 10.2210/pdb3j4a/pdb
• 関連するPDBエントリー: 3J4B
• EMDBエントリー: 5690
• 分子名称: Head-to-tail joining protein (1 entity in total)
• 機能のキーワード: bacteriophage, dna translocation, connector, viral protein
• 由来する生物種: Enterobacteria phage T7
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 666551.06

構造登録者

Cuervo, A.,Pulido-Cid, M.,Chagoyen, M.,Arranz, R.,Gonzalez-Garcia, V.A.,Garcia-Doval, C.,Caston, J.R.,Valpuesta, J.M.,van Raaij, M.J.,Martin-Benito, J.,Carrascosa, J.L. (登録日: 2013-07-09, 公開日: 2013-08-07, 最終更新日: 2024-02-21)

主引用文献

Cuervo, A.,Pulido-Cid, M.,Chagoyen, M.,Arranz, R.,Gonzalez-Garcia, V.A.,Garcia-Doval, C.,Caston, J.R.,Valpuesta, J.M.,van Raaij, M.J.,Martin-Benito, J.,Carrascosa, J.L.
Structural characterization of the bacteriophage t7 tail machinery.
J.Biol.Chem., 288:26290-26299, 2013

PubMed Abstract: Most bacterial viruses need a specialized machinery, called "tail," to inject their genomes inside the bacterial cytoplasm without disrupting the cellular integrity. Bacteriophage T7 is a well characterized member of the Podoviridae family infecting Escherichia coli, and it has a short noncontractile tail that assembles sequentially on the viral head after DNA packaging. The T7 tail is a complex of around 2.7 MDa composed of at least four proteins as follows: the connector (gene product 8, gp8), the tail tubular proteins gp11 and gp12, and the fibers (gp17). Using cryo-electron microscopy and single particle image reconstruction techniques, we have determined the precise topology of the tail proteins by comparing the structure of the T7 tail extracted from viruses and a complex formed by recombinant gp8, gp11, and gp12 proteins. Furthermore, the order of assembly of the structural components within the complex was deduced from interaction assays with cloned and purified tail proteins. The existence of common folds among similar tail proteins allowed us to obtain pseudo-atomic threaded models of gp8 (connector) and gp11 (gatekeeper) proteins, which were docked into the corresponding cryo-EM volumes of the tail complex. This pseudo-atomic model of the connector-gatekeeper interaction revealed the existence of a common molecular architecture among viruses belonging to the three tailed bacteriophage families, strongly suggesting that a common molecular mechanism has been favored during evolution to coordinate the transition between DNA packaging and tail assembly.

PubMed: 23884409
DOI: 10.1074/jbc.M113.491209

実験手法

ELECTRON MICROSCOPY (12 Å)