3J1A

HK97-like fold fitted into 3D reconstruction of bacteriophage CW02

3J1A の概要

• エントリーDOI: 10.2210/pdb3j1a/pdb
• EMDBエントリー: 5388
• 分子名称: capsid protein (1 entity in total)
• 機能のキーワード: halophage, bacteriophage hk97, bacteriophage t7, t7-like phage, turret, extremophile, great salt lake, virus
• 由来する生物種: Great Salt Lake bacteriophage CW02
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 153612.73

構造登録者

Shen, P.S.,Domek, M.J.,Sanz-Garcia, E.,Makaju, A.,Taylor, R.,Culumber, M.,Breakwell, D.P.,Prince, J.T.,Belnap, D.M. (登録日: 2012-01-31, 公開日: 2012-05-30, 最終更新日: 2024-02-21)

主引用文献

Shen, P.S.,Domek, M.J.,Sanz-Garcia, E.,Makaju, A.,Taylor, R.M.,Hoggan, R.,Culumber, M.D.,Oberg, C.J.,Breakwell, D.P.,Prince, J.T.,Belnap, D.M.
Sequence and Structural Characterization of Great Salt Lake Bacteriophage CW02, a Member of the T7-Like Supergroup.
J.Virol., 86:7907-7917, 2012

PubMed Abstract: Halophage CW02 infects a Salinivibrio costicola-like bacterium, SA50, isolated from the Great Salt Lake. Following isolation, cultivation, and purification, CW02 was characterized by DNA sequencing, mass spectrometry, and electron microscopy. A conserved module of structural genes places CW02 in the T7 supergroup, members of which are found in diverse aquatic environments, including marine and freshwater ecosystems. CW02 has morphological similarities to viruses of the Podoviridae family. The structure of CW02, solved by cryogenic electron microscopy and three-dimensional reconstruction, enabled the fitting of a portion of the bacteriophage HK97 capsid protein into CW02 capsid density, thereby providing additional evidence that capsid proteins of tailed double-stranded DNA phages have a conserved fold. The CW02 capsid consists of bacteriophage lambda gpD-like densities that likely contribute to particle stability. Turret-like densities were found on icosahedral vertices and may represent a unique adaptation similar to what has been seen in other extremophilic viruses that infect archaea, such as Sulfolobus turreted icosahedral virus and halophage SH1.

PubMed: 22593163
DOI: 10.1128/JVI.00407-12

実験手法

ELECTRON MICROSCOPY (16 Å)