3IZO

Model of the fiber tail and its interactions with the penton base of human adenovirus by cryo-electron microscopy

3IZO の概要

• エントリーDOI: 10.2210/pdb3izo/pdb
• EMDBエントリー: 7034
• 分子名称: Penton protein, Fiber (2 entities in total)
• 機能のキーワード: human adenovirus fiber tail, pentameric penton base, trimeric fiber, viral protein
• 由来する生物種: Human adenovirus 5 (HAdV-5); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 501682.21

構造登録者

Liu, H. (登録日: 2010-11-05, 公開日: 2011-01-05, 最終更新日: 2024-05-15)

主引用文献

Liu, H.,Wu, L.,Zhou, Z.H.
Model of the Trimeric Fiber and Its Interactions with the Pentameric Penton Base of Human Adenovirus by Cryo-electron Microscopy.
J.Mol.Biol., 406:764-774, 2011

PubMed Abstract: Adenovirus invades host cells by first binding to host receptors through a trimeric fiber, which contains three domains: a receptor-binding knob domain, a long flexible shaft domain, and a penton base-attachment tail domain. Although the structure of the knob domain associated with a portion of the shaft has been solved by X-ray crystallography, the in situ structure of the fiber in the virion is not known; thus, it remains a mystery how the trimeric fiber attaches to its underlying pentameric penton base. By high-resolution cryo-electron microscopy, we have determined the structure of the human adenovirus type 5 (Ad5) to 3.6-Å resolution and have reported the full atomic models for its capsid proteins, but not for the fiber whose density cannot be directly interpreted due to symmetry mismatch with the penton base. Here, we report the determination of the Ad5 fiber structure and its mode of attachment to the pentameric penton base by using an integrative approach of multi-resolution filtering, homology modeling, computational simulation of mismatched symmetries, and fitting of atomic models into cryo-electron microscopy density maps. Our structure reveals that the interactions between the trimeric fiber and the pentameric penton base are mediated by a hydrophobic ring on the top surface of the penton base and three flexible tails inserted into three of the five available grooves formed by neighboring subunits of penton base. These interaction sites provide the molecular basis for the symmetry mismatch and can be targeted for optimizing adenovirus for gene therapy applications.

PubMed: 21146538
DOI: 10.1016/j.jmb.2010.11.043

実験手法

ELECTRON MICROSCOPY (3.6 Å)